| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,832,718 | 0 | T | G | 56.2% | 10.5 / 17.5 | 16 | intergenic (‑95/‑165) | ascG/ascF | DNA‑binding transcriptional regulator AscG/PTS cellobiose/arbutin/salicin transporter subunit IIBC |
| Reads supporting (aligned to +/- strand): ref base T (2/5); new base G (9/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAA > NZ_CP009273/2832650‑2832801 | aCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGac < 2:446675/90‑2 (MQ=255)aCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGaa < 2:251475/90‑1 (MQ=255)aCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGaa > 1:404235/1‑90 (MQ=255)aCGCAGAGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGACCACAAAAATAAAAAATGaa > 1:269190/1‑90 (MQ=255) tACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCAc > 1:193192/1‑90 (MQ=255) gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATTTAAAAAATGAACAATTCActctc > 1:151209/1‑90 (MQ=255) ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAAAGAACAATTCACTCTCTTg > 1:128521/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGGAAAAAATTTAGGGGACCGGGTTCACAAATATAAAAAAAGAACAAATCACTCTCTTTCt > 2:355908/1‑90 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATTTAAAAAATGAACAATTCACTCTCTTGCt < 2:151209/90‑1 (MQ=255) gTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt > 1:135269/1‑90 (MQ=255) tGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTCAGGGGGCCCGGTTCCCAAATATAAAAAAAGAAGAAATAACACtctttctttt > 2:460411/1‑88 (MQ=255) gTCCTTGCACAATCGGCGGGAAAAATATTCAGGGGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGaa > 1:388173/1‑89 (MQ=255) tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGCCCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACa < 2:340425/90‑1 (MQ=255) cctGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc < 2:118146/88‑1 (MQ=255) ttGCACAATCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACt > 1:397574/1‑90 (MQ=255) gACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTaa > 2:258749/1‑90 (MQ=255) | ACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAA > NZ_CP009273/2832650‑2832801 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |