| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,897,070 | 0 | C | A | 55.6% | 2.9 / 11.6 | 9 | intergenic (+337/+1036) | ygcE/queE | FGGY‑family carbohydrate kinase/7‑carboxy‑7‑deazaguanine synthase QueE |
| Reads supporting (aligned to +/- strand): ref base C (0/4); new base A (5/0); total (5/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTC‑AAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAA > NZ_CP009273/2896989‑2897144 | aaCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTC‑AAAACAAAGACaa < 1:482540/90‑1 (MQ=255) tCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACAACCATTTACCTCTAAAAAAAAAAAAAAATTCCaa > 2:499019/1‑89 (MQ=255) tAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACCCCTTAACCTC‑AAAAAAAAAACAAAAATCCCC‑ATTTATTACtt > 1:308848/1‑90 (MQ=255) aTTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCATTTACCCC‑AAAAAAAAAAAAAAAATCCAGAATTTTTTATTTAACaaaa > 2:59247/1‑89 (MQ=255) tcaACAGCGTATAGAGGCGGTTTTTTAAAAACACTCATTAGCCCC‑AAAAAAAAAACAAAATTCCCG‑ATTTTTTTCTTTAACAAAAAATaa > 1:256490/1‑90 (MQ=255) gCGTATAGAGGCGGGTATGTAAAAACACCCCTTAACCTC‑AAAAAAAAAAAAAAAATCCAC‑ATTTATTTTTTAACCAAAAAATAaaaaaat > 2:295196/1‑87 (MQ=255) ggTTATGTAAAACCACTCATTAGCCTC‑AAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATAc < 2:168320/90‑1 (MQ=255) aCCACTCATTAGCCTC‑AAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTaa < 1:245070/90‑1 (MQ=255) tCATTAGCCTC‑AAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaa < 2:274433/90‑1 (MQ=255) | AACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTC‑AAAACAAAGACAAACTTCCAG‑ATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAA > NZ_CP009273/2896989‑2897144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |