| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,175,957 | 0 | A | T | 55.6% | ‑0.6 / 16.3 | 18 | I17F (ATT→TTT) | zupT | zinc transporter ZupT |
| Reads supporting (aligned to +/- strand): ref base A (2/6); new base T (10/0); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAT > NZ_CP009273/3175870‑3176036 | aCGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGGTtttt > 1:59594/1‑90 (MQ=255) gAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTTGCGCGGTTTTCTGCgg > 1:445918/1‑89 (MQ=255) gAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGTTTATTTGCGCGTTTTTCCGCGGTTT‑CGGGCaaaaa > 1:339097/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCGTTTTTTTGGGCGTTTTTCCCCGTTCT‑CCGGGaaaaaac > 1:451891/1‑88 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCCCCATTTTTTGCGGGCTTTTCCGCCGTCT‑CCGGCAAAAAcc > 2:92935/1‑90 (MQ=255) gTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGGAGCCACGGTTTTTTGGGCGGTTTTCGGCGTTTT‑CGGGCaaaaaac > 1:484453/1‑88 (MQ=255) tGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCt < 1:77514/90‑1 (MQ=255) gTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTTTTTGCGCGGTTCTCGGCGGTCT‑CCGGCAAAAAacccc > 1:93018/1‑88 (MQ=255) tGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCCCGTTTTTTTGCGCGGTTTTCGGCGGTCT‑CGGGCAAAAAACCCCCAAcc > 1:342859/1‑90 (MQ=255) tCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCCAAAACCCCCGAACCGc > 1:134426/1‑90 (MQ=255) tctcATTCTGACCATACTGGCGGGGGGAGCCCCGGTTTTTTGGGCGGTTTTTCGCGTTCTCCGGGCAAAAACCC‑CCCACCGCTTTCTggg > 2:383178/1‑89 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCCGCCACGTTTTTTTGCGCGGTTTTCGGCGTTTT‑CCGGGAAAAAACCTCGAACCGCTTCCTggg > 2:14963/1‑89 (MQ=255) tctcATTCTGACCATACTGGCGGGGGCAGCCCCGTTTTTTGGGGCGTTTTTCGGCGTTTT‑CGGGCAAAAACCCCCCAACCCCTTACTGGc > 1:425556/1‑90 (MQ=255) cATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGtt < 2:451891/90‑1 (MQ=255) gACCATACTGGCGGGGGGAGCCCCGTTTTTTTGGGCGTTTTTCTGCGTTCTCCGGGCAAAAACCCCCAACCCCCTTCTTGGGGttttgct > 2:437478/1‑87 (MQ=255) aCCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 1:247984/90‑1 (MQ=255) aCCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTa < 2:210255/90‑1 (MQ=255) gggggCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTgcggc < 1:92935/90‑1 (MQ=255) aGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAt < 1:383178/90‑1 (MQ=255) | ACGATCTGCCTGAAGGTGAAGATTTATAAGGAGTTGTCGATGTCAGTACCTCTCATTCTGACCATACTGGCGGGGGCAGCCACGTTTATTGGCGCGTTTCTCGGCGTTCT‑CGGGCAAAAACCCTCGAACCGCTTACTGGCGTTTTCGCTAGGTTTTGCGGCGGGGAT > NZ_CP009273/3175870‑3176036 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |