| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,061,105 | 0 | A | G | 55.6% | 3.2 / 18.6 | 18 | L511P (CTG→CCG) | yihQ | sulfoquinovosidase |
| Reads supporting (aligned to +/- strand): ref base A (6/2); new base G (0/10); total (6/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACCTTCGTGGGTGCGCATCATCGGCGTGAAGGCGCTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACC > NZ_CP009273/4061019‑4061182 | tACCTTCGTGGGTGCGCATCATCGGCGTGAAGGCGCTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAggg > 1:126061/1‑90 (MQ=255) aCCTTCGTGGGTGCGCATCATCGGCGTGAAGGCGCTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGggt < 2:64380/90‑1 (MQ=255) aCCTTCGTGGGTGCGCATCATCGGCGTGAAGGCGCTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGggt < 1:951/90‑1 (MQ=255) gcgcATCATCGGCGTGAAGGCGCTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGcc > 2:24380/1‑90 (MQ=255) tcGGCGTGAGGGCGCGGAAACCGCCCCCGCGCAGCAGCAGCTTTTTGCGGCGCTCCATCCAAAACGGGGTGGTGTAACCGCCAATGTCGc < 2:372644/90‑1 (MQ=255) ggCGTGAAGGCGCTAAAATCCCCCCAGCGCAGCAGCACCTCTTTGCTGCGCTTCATCCAAAACGGGGTGGTGTAACCGCCAATGTCGCtg < 1:340209/90‑1 (MQ=255) ggCGTGAAGGCGCTAAAACCCCCCCAGCGCAGCAGCAGCTTTTTGCTGCGCTTCATCACAAACGGGGTGGTGTAACCGCCAATGTCGCtg < 1:262276/90‑1 (MQ=255) gCGTGAAGGCGCTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCCCtgt > 2:476648/1‑90 (MQ=255) gTGAAGGCGCTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCGCTgtgg > 1:304939/1‑90 (MQ=255) agggCGCTAAAACCGCCCCGGCGCAGCAGCAGCTTTTTGCTGCGCTTCATCCCAAACGGGGTGGTGAAACCGCCAATGTCGCTGTGGTGc < 2:67139/88‑1 (MQ=255) agggCGCTAAAACCGCACCGGCGCAGCAGCAGCTTTTTGCGGCGCTTCATCTAAAAGGGGGTGGTGTAACCGCCAATGTCGCTGTGGTGc < 2:217842/88‑1 (MQ=255) gcTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGcc > 2:446385/1‑90 (MQ=255) gCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGt > 1:39796/1‑90 (MQ=255) ccggcgcACCAGCAGTTTTTTGCTGCGTTTCATCTAAAACGGGGGGGTGAAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCAt < 1:416421/87‑1 (MQ=255) ccAGCGCAGCAGCAGCTTTTTGCTGCGCTTCATCCCAAACGGGGTGGTTTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCAt < 1:447793/90‑1 (MQ=255) gcagcagcgGCTTTTTGCGGCGCTTCACCAAAAAGGGGGTGGTGTACCCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCa < 2:153786/90‑1 (MQ=255) ttGCGGCGCTTCATCTAAAACGGGGGGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCgccgcc < 1:66776/90‑1 (MQ=255) ttCCTCCCAAAAGGGGGGGTGAAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAAcc < 1:252220/90‑1 (MQ=255) | TACCTTCGTGGGTGCGCATCATCGGCGTGAAGGCGCTGAAATCGCACCAGCGCAGCAGCAGCTCTTTGCTGCGCTTCATCTCAAACAGGGTGGTGTAACCGCCAATGTCGCTGTGGTGCAGGCCATGTCCGGTCATTGCCAGCGACAGCGCCGCCGGGACAACC > NZ_CP009273/4061019‑4061182 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |