| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 687,303 | 0 | A | G | 54.5% | 10.9 / 28.1 | 22 | intergenic (‑63/+27) | corC/ybeY | CNNM family magnesium/cobalt transport protein CorC/rRNA maturation RNase YbeY |
| Reads supporting (aligned to +/- strand): ref base A (0/10); new base G (12/0); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.55e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTC > NZ_CP009273/687220‑687383 | gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 1:441459/1‑90 (MQ=255)gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTg > 2:217997/1‑90 (MQ=255)gtgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTg > 1:462823/1‑90 (MQ=255) tgtgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGc > 2:271401/1‑90 (MQ=255) tgAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCa < 1:271401/90‑1 (MQ=255) tcgtcgCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGTCaaaa > 1:239542/1‑89 (MQ=255) cTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTgg < 1:371644/90‑1 (MQ=255) ggTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCCGGCAAAAGCCCGGCAAATTATTTTTtttc > 1:241036/1‑90 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCAAAAGCCCGGCAAAATATTTTTTTTCGGc > 2:107614/1‑90 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAATGCCTGGCAAATTATTTTTTTTCGGc > 1:479164/1‑90 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc < 1:81299/90‑1 (MQ=255) tctcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACATCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGc < 2:156059/90‑1 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCAAAAGCCTGGCAAATTATTCTTTTTCGGcca > 2:95321/1‑88 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCAAAAGCCTGGCAAAATATTTTTTTTCGGCaa > 2:186963/1‑90 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGTCTGCCCGTCAAAAACCCGGCAAATTATTTTTTTTCCGCaa > 2:492019/1‑90 (MQ=255) tcATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGGCGGCTGCCAGTCAAAAGCCTGGCAAAATATTTTTTTTCGGCaa > 2:61506/1‑90 (MQ=255) agttaATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACgg < 1:367516/90‑1 (MQ=255) aaaaCGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCAt < 2:216045/90‑1 (MQ=255) gCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGccc < 1:107614/90‑1 (MQ=255) cgtTCATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGGAAATTATTCTTTCTCGGCAACGTACGGATCCTCATAGCCCAGagc < 2:412796/90‑1 (MQ=255) cgtTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGagc < 2:239542/90‑1 (MQ=255) aCGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAAtctc < 1:61506/90‑1 (MQ=255) | GTGTGAATTGTCGTCGCTCATGGCGTCGTATGGGTTCTCATGTTAGTTAATCAAAACGCCGTCGTTAATCACCAACGGCGGGGACGTCTGCCAGTCAAATGCCTGGCAAATTATTCTTTCTCGGCAATGTACGGATCCTCATAGCCCAGAGCAAGCATAATCTC > NZ_CP009273/687220‑687383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |