Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,607,750	G→A	G204D (GGC→GAC)	deoC →	deoxyribose‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,607,750	0	G	A	100.0%	79.7 / NA	22	G204D (GGC→GAC)	deoC	deoxyribose‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/12);  total (10/12)

TGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTC  >  NZ_CP009273/4607663‑4607833                                                                                        |                                                                                    tgAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCggcggacg                                                                                   <  1:325804/90‑1 (MQ=255) tgAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCggcggacg                                                                                   <  1:287619/90‑1 (MQ=255)      gcgACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGgcgtg                                                                              <  1:182694/90‑1 (MQ=255)       cgACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGgcgtgc                                                                             >  1:199776/1‑90 (MQ=255)                       gcgcATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATgc                                                             <  2:432386/90‑1 (MQ=255)                                    gAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTAc                                                                         >  1:421573/1‑65 (MQ=255)                                    gAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTAc                                                                         <  2:421573/65‑1 (MQ=255)                                    gAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATAtctc                                                >  1:23307/1‑90 (MQ=255)                                         gATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCAt                                           >  1:215275/1‑90 (MQ=255)                                         gATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCAt                                           >  1:265496/1‑90 (MQ=255)                                          aTCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCAtt                                          <  2:320623/90‑1 (MQ=255)                                          aTCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCAtt                                          <  2:236392/90‑1 (MQ=255)                                              gTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAg                                      >  1:293402/1‑90 (MQ=255)                                                gATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGAt                                    >  2:343273/1‑90 (MQ=255)                                                   atGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGaa                                 >  1:13190/1‑90 (MQ=255)                                                    tGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAAc                                <  1:284032/90‑1 (MQ=255)                                                    tGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAAc                                <  1:191494/90‑1 (MQ=255)                                                     gggCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACt                               >  2:168884/1‑90 (MQ=255)                                                         gTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTc                           <  2:68728/90‑1 (MQ=255)                                                         gTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTc                           >  1:24971/1‑90 (MQ=255)                                                         gTAGAAAAAACCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTc                           <  1:223105/90‑1 (MQ=255)                                                             aaaaaaCCGTTGGTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTg                       <  1:181202/90‑1 (MQ=255)                                                                        ggTTTCAAACCGGCGGACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCa            <  2:316980/90‑1 (MQ=255)                                                                                  cggcggACGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTc  <  2:13190/90‑1 (MQ=255)                                                                                        |                                                                                    TGAACGCGACGCCGGAAAGCGCGCGCATCATGATGGAAGTGATCCGTGATATGGGCGTAGAAAAAACCGTTGGTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATCTCGCCATTGCAGATGAACTGTTCGGTGCTGACTGGGCAGATGCGCGTC  >  NZ_CP009273/4607663‑4607833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑