Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	264,689	(A)5→4	intergenic (‑18/+311)	ykfA ← / ← perR	GTPase family protein/LysR substrate‑binding domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	264,685	0	A	.	100.0%	41.8 / NA	11	intergenic (‑14/+315)	ykfA/perR	GTPase family protein/LysR substrate‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/7);  total (4/7)

CATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGT  >  NZ_CP009273/264613‑264744                                                                         |                                                            cATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGt                                           >  2:9309/1‑90 (MQ=255)   ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATaaaaa                                                >  1:111632/1‑83 (MQ=255)   ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATaaaaa                                                <  2:111632/83‑1 (MQ=255)   ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAg                                         >  1:196529/1‑90 (MQ=255)     gCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGaa                                       >  2:203354/1‑90 (MQ=255)         cAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGt                                   <  2:238484/90‑1 (MQ=255)                               aaCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt             <  2:143848/90‑1 (MQ=255)                               aaCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt             <  2:53183/90‑1 (MQ=255)                                       cAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCag     <  2:196529/90‑1 (MQ=255)                                         aaCCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCagag   <  2:153200/90‑1 (MQ=255)                                          aCCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGt  <  2:204949/90‑1 (MQ=255)                                                                         |                                                            CATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGT  >  NZ_CP009273/264613‑264744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑