Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,076,727	(T)5→4	intergenic (+458/‑85)	putP → / → BW25113_RS05295	sodium/proline symporter PutP/FTR1 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,076,723	0	T	.	100.0%	35.6 / NA	10	intergenic (+454/‑89)	putP/BW25113_RS05295	sodium/proline symporter PutP/FTR1 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (4/6);  total (4/6)

CTGGAATAATCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAA  >  NZ_CP009273/1076643‑1076793                                                                                 |                                                                       cTGGAATAATCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTG‑TTTTCTTTGc                                                              >  2:27732/1‑90 (MQ=255)            gTTTAGCTCCCTTGGCATTTCCCCTCTTTGTTTGCATTCCAACATCATTTTATAAACATTCCGCTTGTG‑TTTTGTTTGCCGTAAGGATaa                                                   <  1:318107/90‑1 (MQ=255)                      cTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATCTTATAAACATTCCGCTTGTG‑TTTTCTTTGCCGTAATGATAATCGCTATCAc                                         <  1:18295/90‑1 (MQ=255)                       ttGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTG‑TTTTCTTTGCCg                                                            <  1:180427/70‑1 (MQ=255)                       ttGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTG‑TTTTCTTTGCCg                                                            >  2:180427/1‑70 (MQ=255)                           cATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTG‑TTTTCTTTGCCGTAATGATAATCGCTATCACTGCGa                                    <  2:348133/90‑1 (MQ=255)                                   ctctTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTG‑TTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACttt                            >  2:189183/1‑90 (MQ=255)                                            tACATTCCAGCATCATTTTATAAACATTCCGCTTGTG‑TTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCAt                   <  2:212839/90‑1 (MQ=255)                                                          atTTTATAAACATTCCGCTTGTG‑TTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGaa     >  2:246174/1‑90 (MQ=255)                                                             ttATAAACATTCCGCTTGTG‑TTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGaaaaa  <  1:246174/90‑1 (MQ=255)                                                                                 |                                                                       CTGGAATAATCGTTTATATCCCTTGGCATTACCTCTCTTTGTTTACATTCCAACATCATTTTATAAACATTCCGCTTGTGTTTTTCTTTGCCGTAATGATAATCGCTATCACTGCGATTTACTTTTCTTTGCATAGATTGACTCAGAAAAA  >  NZ_CP009273/1076643‑1076793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑