Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,276,251	Δ1 bp	coding (932/3744 nt)	narG →	nitrate reductase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,276,249	0	G	.	83.3%	11.9 / ‑3.7	6	coding (930/3744 nt)	narG	nitrate reductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (3/2);  total (4/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACC  >  NZ_CP009273/1276162‑1276303                                                                                        |                                                       cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggc                                                      <  1:334578/90‑2 (MQ=255) cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggc                                                      <  2:334311/90‑2 (MQ=255)            aCGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAAtgct                                          >  1:254405/1‑90 (MQ=255)                                 gCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGA‑GGGCCACGGAATGCGGCGTGAATTCCACCTCGACaa                     >  2:280060/1‑90 (MQ=255)                                         tggctggcACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCAGGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGcc             <  2:154122/90‑1 (MQ=255)                                                    gAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCAcc  >  2:57459/1‑90 (MQ=255)                                                                    gCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAAtt                                 >  1:275410/1‑43 (MQ=38)                                                                    gCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAAtt                                 <  2:275410/43‑1 (MQ=38)                                                                                        |                                                       CACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACC  >  NZ_CP009273/1276162‑1276303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑