Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	508,601	(C)6→7	coding (570/1293 nt)	ybaT →	APC family permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	508,595	1	.	C	100.0%	20.9 / NA	8	A188A (GCG→GCC)	ybaT	APC family permease
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (4/4);  total (4/4)

CCTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCG‑CCCCCCAGCTCCGGTGCGTTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCG  >  NZ_CP009273/508508‑508679                                                                                         |                                                                                     ccTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCG‑cc                                                                                    <  1:156630/90‑1 (MQ=255)                      gatCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCCAGCTCCGGTGCGttct                                                                >  1:21639/1‑90 (MQ=255)                      gatCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCCAGCTCCGGTGCGttct                                                                <  1:39591/90‑1 (MQ=255)                        tCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCCAGCTCCGGTGCGttcttc                                                              <  1:332163/90‑1 (MQ=255)                                 tGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCCAGCTCCGGTGCGTTCTTCTCCTGTAtt                                                     >  2:140495/1‑90 (MQ=255)                                 tGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCCAGCTCCGGTGCGTTCTTCTCCTGTAtt                                                     >  2:272298/1‑90 (MQ=255)                                     gATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCCAGCTCCGGTGCGTTCTTCTCCTGTATTGGGa                                                 <  2:254446/90‑1 (MQ=255)                                         attGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCGCCCCCCCAGCTCCGGTGCGTTCTTCTCCTGTATTGGGATAAc                                             <  2:321027/90‑1 (MQ=255)                                                                                    ctGCGCCCCCCCAGCTCCGGTGCGTTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACgcggcg  >  1:327470/1‑90 (MQ=255)                                                                                         |                                                                                     CCTCGTCGGCATTAAAATGATGATCCTGTTATTGCTGATTATTGCCGGTGTCTGGTCGCTGCAACCGGCGCATATTTCCGTCTCTGCG‑CCCCCCAGCTCCGGTGCGTTCTTCTCCTGTATTGGGATAACTTTCCTTGCCTATGCGGGCTTTGGCATGATGGCGAACGCGGCG  >  NZ_CP009273/508508‑508679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑