Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273499,3160AC58.3% 7.5 / 11.9 12G279G (GGT→GGG) fsrfosmidomycin MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/7);  total (5/7)
Fisher's exact test for biased strand distribution p-value = 1.26e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GAGGATAGAGCCCCAAATCACATATTTCCGCCCAA‑TTTT‑ATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTG  >  NZ_CP009273/499250‑499403
                                                                    |                                                                                       
gAGGATAGAGCCCCAAATCACATATTTCCGCCCAA‑TTTT‑ATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGaa                                                                  >  2:330659/1‑90 (MQ=255)
      agcccccaAAACCACAT‑TTCCCCCCCAATTTTT‑CCCCCCAACGGCCCCGCCGATCACCGTCCCTGCCGCAACGGCAAACAGGAAGGCaaa                                                            <  1:110762/86‑1 (MQ=255)
       gagCCCCAAATCACATATTTCCGCCCAA‑TTTT‑ATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAAc                                                           >  2:110762/1‑90 (MQ=255)
       agaccccAAAACCCATTTTCCCCCCCAA‑TTTTCCCCCCCCACGGCCCCCCCGACCCCCTTCCCTGCCGCAACGGCAAACAGGAAGGCaaa                                                            <  1:330659/87‑1 (MQ=255)
              aaaTCACATATTTCCGCCCAA‑TTTT‑ATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAGACAGATGaa                                                    >  2:44393/1‑90 (MQ=255)
                                           ccccAACGGCCCCGCCGATCCCCGTCCCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGa                         <  1:103652/90‑1 (MQ=255)
                                              cTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAAtt                      >  1:318568/1‑90 (MQ=255)
                                                      ccgccgCTCCCCGTCCCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATc              <  2:318568/90‑1 (MQ=255)
                                                      ccgccaACCCCCGTCCCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATc              <  1:149711/90‑1 (MQ=255)
                                                            aTCCCCGTCCCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATaa        <  1:241885/90‑1 (MQ=255)
                                                              cACCTTCCCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATaaaa      <  1:117126/90‑1 (MQ=255)
                                                                  gTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGgtg  >  2:172939/1‑90 (MQ=255)
                                                                    |                                                                                       
GAGGATAGAGCCCCAAATCACATATTTCCGCCCAA‑TTTT‑ATCCCCTACAGGCCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTG  >  NZ_CP009273/499250‑499403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑