| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 575,614 | 0 | A | G | 63.2% | 6.5 / 17.7 | 19 | intergenic (+72/+93) | BW25113_RS02885/ylcI | hypothetical protein/YlcI/YnfO family protein |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base G (7/5); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCG > NZ_CP009273/575528‑575700 | cTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAg > 1:124987/1‑90 (MQ=255) ggtggtTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTg > 2:35212/1‑90 (MQ=255) ggtTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTa > 2:264378/1‑90 (MQ=255) tCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAAc < 2:124158/90‑1 (MQ=255) ttCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCt < 1:18501/90‑1 (MQ=255) ctactTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAAt > 1:191738/1‑90 (MQ=255) aaCCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTg < 1:114683/90‑1 (MQ=255) ccTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGaa > 2:224791/1‑90 (MQ=255) gACAGAGCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTAc < 2:316087/90‑1 (MQ=255) gACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTAc < 1:35212/90‑1 (MQ=255) gagCCAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAata > 2:211739/1‑90 (MQ=255) cAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTg > 2:107497/1‑75 (MQ=255) cAACACGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTg < 1:107497/75‑1 (MQ=255) acacGCAGTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTcc > 2:215/1‑90 (MQ=255) gTCTGTCACTGTCAGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCgg < 2:191738/90‑1 (MQ=255) cactgtcaGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGgaa < 1:215/90‑1 (MQ=255) gtcaGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAAc > 1:205188/1‑90 (MQ=255) caGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAAcgc > 2:162932/1‑90 (MQ=255) aGGGAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAAcgcg < 2:205188/90‑1 (MQ=255) | CTGGTGGTTGACTGATCACCATAACTGCTAATCATTCAAACTACTTAACCTGTGACAGAGCCAACACGCAGTCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATTAAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCG > NZ_CP009273/575528‑575700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |