Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,348,7170GC54.5% ‑6.4 / 13.7 11G325A (GGC→GCC) glpBglycerol‑3‑phosphate dehydrogenase subunit GlpB
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base C (3/3);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGC  >  NZ_CP009273/2348657‑2348800
                                                            |                                                                                   
gTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGAtg                                                        <  1:219962/90‑1 (MQ=255)
gTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGAtg                                                        <  2:229591/90‑1 (MQ=255)
gTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGAtg                                                        <  2:96673/90‑1 (MQ=255)
  ttCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGCCATTCGAGAGCCGATtctc                                                                  <  1:99031/78‑1 (MQ=255)
  ttCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGCCATTCGAGAGCCGATtctc                                                                  >  2:99031/1‑78 (MQ=255)
                  gCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGCCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCAcgc                                      >  2:224179/1‑90 (MQ=255)
                                  tGGCGGAGTGTTAGCGGAACGTAACGCCATTTGAGATCCTATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATaa                      <  1:303558/90‑1 (MQ=255)
                                           ggTAGCGGAACGTAACGCCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAAt                             <  1:210230/74‑1 (MQ=255)
                                           ggTAGCGGAACGTAACGCCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAAt                             >  2:210230/1‑74 (MQ=255)
                                                 ggAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTgc       >  2:274655/1‑90 (MQ=255)
                                                      gTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGcgccgc  >  1:291714/1‑90 (MQ=255)
                                                            |                                                                                   
GTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGC  >  NZ_CP009273/2348657‑2348800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑