| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,840,042 | 0 | G | T | 57.1% | 9.6 / 18.0 | 14 | Q244K (CAA→AAA) | hycD | formate hydrogenlyase subunit HycD |
| Reads supporting (aligned to +/- strand): ref base G (0/6); new base T (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGC > NZ_CP009273/2839965‑2840131 | cAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTTCCTTTTTCCCCCCGGaaa > 2:306044/1‑89 (MQ=255) ggACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGGTTCCATTTTTCCCCCCCGAATaa > 2:152861/1‑90 (MQ=255) ccaGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCcgacga < 2:50727/90‑1 (MQ=255) caGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGaa < 1:152861/90‑1 (MQ=255) tACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTTTCCCCCCGGAATAAAAACCCCGACGAACATCtt > 2:29569/1‑89 (MQ=255) gATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCaa < 2:32419/90‑1 (MQ=255) ggCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGGTTCCATTTTTCCCCCCGGAATAAAAACCCCCACGAAAATCTGCAacac > 2:222928/1‑90 (MQ=255) ggCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGGTTCCATTTTTCCCCCCGGAAAAAAAACCCCCACGAACATCTGCAacac > 2:37212/1‑90 (MQ=255) ggCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGGGAAGGGTTTCATTTTTCCCCCCGGAAAAAAAACCCCGCCGAACATCTGCAacac > 1:276589/1‑90 (MQ=255) agcagcagTCCACCGGCGGTGAAGGGTTCCATTTTTCCCCCCGGAAAAAAAACCCCCACGAACATCTGCAACACCCCCAGCTGTTTTAgg > 2:200102/1‑90 (MQ=255) agcagcagTCCACCGGCGGTGAAAGGTTCCATTTTTCCCCACGGAATAAAAACCCCCACGAAAATCTGCAAAACCACCAGCTGTTTCaag > 2:218212/1‑88 (MQ=255) ccGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCAt < 1:61019/90‑1 (MQ=255) gTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATg < 1:222928/90‑1 (MQ=255) gTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATg < 2:202271/90‑1 (MQ=255) gTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAgccgctgc < 2:72521/90‑1 (MQ=255) | CAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGC > NZ_CP009273/2839965‑2840131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |