| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,779,872 | 0 | T | G | 60.0% | 8.4 / 10.5 | 15 | V418G (GTT→GGT) | gpmM | 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (9/0); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.10e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCA > NZ_CP009273/3779786‑3779943 | cGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGa < 1:61320/90‑1 (MQ=255) ggCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGGTGaa > 2:146086/1‑90 (MQ=255) cAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAgc < 1:202984/90‑1 (MQ=255) aTCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAATGGGTTGAAGCGCTGGGTCACTGCGTGGGAGAAGAcg > 2:166389/1‑90 (MQ=255) aTCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAACGGGTGAAGCGCTGGGTCACTGCGTGGGAGAAGGcg > 2:172291/1‑90 (MQ=255) aTCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAACGGGTGAAGCGCTGGGTCACTGCGTGGGAGAAGGcg > 2:260524/1‑90 (MQ=255) aTCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAACGGGTGAAGCGCTGGGTCACTGCGGGGGAGAAGGcg > 2:288784/1‑90 (MQ=255) aTCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGGTAAAAGGGGTGAAGCGCTGGATCACTGCGGGggagaaagcg > 2:213670/1‑86 (MQ=255) aCATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTg < 1:7580/90‑1 (MQ=255) aGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGGTGAAGCGCTGGATCACTGCGGGGGAGAAGGCGCGAAAGCGGGTGAGTCCgg > 1:71649/1‑89 (MQ=255) cacaCCGGGGTGATGGAAGCGGCGGTTAAAACGGGTGAAGAGCCGGGTCACTGCGGGGGAAAAGACGCGAAAACGGGTGAGTGCgggggg > 1:46690/1‑89 (MQ=255) acacCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGGGGGAGAAGTCGCGAAAGCGGGTGAGTCCggggggg > 2:41392/1‑88 (MQ=255) cGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACa < 1:41392/90‑1 (MQ=255) aTGGAAGCGGCGGTTAAAACGGGTGAAGCGCTGGATCACTGGGTGGGAGAAGGCGCGAAAGGGGGTGGGTCCGGGGGGGGGCAActgctg > 1:10966/1‑90 (MQ=255) aaGCGGCGGTTAAAACGGGTGAAGCGCTGGATAACTGCGGGGGAGAAGGCGCGAAAACGGGGGAGGCCGTGGGGGGGCAAATGCGGATCa > 2:334315/1‑90 (MQ=255) | CGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGCGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCA > NZ_CP009273/3779786‑3779943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |