| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,996,864 | 0 | T | G | 54.5% | 8.5 / 10.2 | 11 | V225V (GTA→GTC) | rarD | EamA family transporter RarD |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATA > NZ_CP009273/3996781‑3996951 | aTCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACCAAAGcgggaccgga > 2:194416/1‑86 (MQ=255) cAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGGACGGGAgg > 2:151620/1‑89 (MQ=255) cGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGGAAAAAACAAAAACGGGACGGGAGGGAc > 1:326528/1‑90 (MQ=255) cTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGGACGGGAGTGACAATACCGgcggcggt > 2:259591/1‑88 (MQ=255) aaGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGt > 2:273810/1‑90 (MQ=255) aaGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGGACGGTAGTGACAATACCGGCGGCGATCAGCAGt > 2:277912/1‑90 (MQ=255) tAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGGACGGTAGGGACAATACCGGCGGCGATCAGCAGGAAAtt > 2:285709/1‑90 (MQ=255) tGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGa < 1:273810/90‑1 (MQ=255) gCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGGACGGGAGGGACAAAACCGGCGGCGGTCAGCAGGAAAATCAGCGACAACgggg > 1:307500/1‑89 (MQ=255) cgTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGccc < 1:66058/90‑1 (MQ=255) aaaaCACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGa < 1:212541/90‑1 (MQ=255) aaCACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGc < 2:307500/90‑1 (MQ=255) ggtacggtaGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATa > 2:343714/1‑90 (MQ=255) | ATCAGCGTCGGGCCAATGTACTGGAAAAAGCCTAACGTTGAGAGACGCAAGCGCGTGGCAGCGGCGGTAAAACACAACAGCGGTACGGTAGTGACAATACCGGCGGCGATCAGCAGTAAATTCAGCGACATCGGGTTTTGCCCCATATGGCTGGTTGAGCTGTCGGCAATA > NZ_CP009273/3996781‑3996951 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |