| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,500,063 | 0 | T | G | 58.3% | 4.8 / 11.4 | 12 | intergenic (+113/+444) | BW25113_RS25830/fecE | hypothetical protein/Fe(3+) dicitrate ABC transporter ATP‑binding protein FecE |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base G (3/4); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCC > NZ_CP009273/4499975‑4500144 | cTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCttt < 1:220373/90‑1 (MQ=255) aaGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTg > 1:232352/1‑90 (MQ=255) gtagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTc > 2:128363/1‑90 (MQ=255) gtagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTc > 1:176760/1‑90 (MQ=255) cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGAtt < 2:35142/90‑1 (MQ=255) aaaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAg > 1:76215/1‑90 (MQ=255) aaaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAg > 1:25628/1‑90 (MQ=255) aGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCAtt < 2:292646/90‑1 (MQ=255) aGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCAtt < 2:303265/90‑1 (MQ=255) tGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAAt < 1:291473/90‑1 (MQ=255) aTGGTTGCTTTTGATTTACGTCGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTc > 2:247557/1‑90 (MQ=255) tGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTcc < 2:24671/90‑1 (MQ=255) tGGTTGCTGTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTcc < 1:101602/90‑1 (MQ=255) | CTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCC > NZ_CP009273/4499975‑4500144 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |