Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,849,315	A→C	Y540S (TAC→TCC)	flhA →	formate hydrogenlyase transcriptional activator FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,849,315	0	A	C	100.0%	20.0 / NA	8	Y540S (TAC→TCC)	flhA	formate hydrogenlyase transcriptional activator FlhA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (1/7);  total (1/7)

GCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTT  >  NZ_CP009273/2849251‑2849399                                                                 |                                                                                     gccgcGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTCCCGCCTGAACGTATTCCCGATTCAc                                                             <  2:31963/90‑1 (MQ=255)         tacccGCGATCTGAAAAAAATGGTCGCCGCCCGTGAGTCCCGTAGCGATCTCTATTCCCGCCTGAACGTATTCCCGATTCACCTgccgcc                                                     <  1:163197/87‑1 (MQ=255)                   ctaaaaaaaaGGGTCGCCGCCCGTGAGTCCCGTAGCGTTCTCTTTCCCCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAg                                           <  1:244700/87‑1 (MQ=255)                                                ccGTGGCGATCTCTATTCCCGCCTGAACGTATCCCCGATTCCCCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGaa              <  1:228433/90‑1 (MQ=39)                                                ccGTAGCGATCTCTATTCCCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGaa              <  2:192866/90‑1 (MQ=255)                                                ccGTAGCGATCTCTATTCCCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGaa              <  2:5694/90‑1 (MQ=255)                                                        aTCTCTTTTCCCGCCTGAACGTATCCCCGATTCCCCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGAAAGCCTTTa      <  1:240608/90‑1 (MQ=39)                                                            ctATTCCCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCtt  >  1:155863/1‑90 (MQ=255)                                                                 |                                                                                     GCCGCGACTAACCGCGATCTGAAAAAAATGGTCGCCGACCGTGAGTTCCGTAGCGATCTCTATTACCGCCTGAACGTATTCCCGATTCACCTGCCGCCACTACGCGAGCGTCCGGAAGATATTCCGCTGCTGGCGAAAGCCTTTACCTT  >  NZ_CP009273/2849251‑2849399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑