Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,535,339	T→C	F525S (TTC→TCC)	feoB →	Fe(2+) transporter permease subunit FeoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,535,339	0	T	C	100.0%	36.2 / NA	13	F525S (TTC→TCC)	feoB	Fe(2+) transporter permease subunit FeoB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/3);  total (10/3)

TTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCG  >  NZ_CP009273/3535255‑3535420                                                                                     |                                                                                  ttAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGa                                                                              >  2:293163/1‑90 (MQ=255)                                       ggCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgtc                                        >  2:171733/1‑90 (MQ=255)                                         cTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGa                                      >  1:233459/1‑90 (MQ=255)                                          ttCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCGACAGCTTCTCGCTGAGCGGGAAAATCGTCGAt                                     >  1:182376/1‑90 (MQ=255)                                                  gCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAAc                                                                   >  1:27490/1‑52 (MQ=255)                                                  gCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAAc                                                                   <  2:27490/52‑1 (MQ=255)                                                        tGGTCAAGTGATCATCATCGTCAGTATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAACGTCGATAACAGCAACGACTc                       <  1:195893/90‑1 (MQ=255)                                                        tGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAAc                                                                   <  1:83092/46‑1 (MQ=255)                                                        tGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAAc                                                                   >  2:83092/1‑46 (MQ=255)                                                          gTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCgg                     >  1:160634/1‑90 (MQ=255)                                                                 gATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGc              >  1:33363/1‑90 (MQ=255)                                                                             cAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCg  >  2:312508/1‑90 (MQ=255)                                                                             cAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCg  >  2:81182/1‑90 (MQ=255)                                                                                     |                                                                                  TTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCG  >  NZ_CP009273/3535255‑3535420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑