Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,896,621	A→G	D147D (GAT→GAC)	bglB ←	6‑phospho‑beta‑glucosidase BglB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,896,621	0	A	G	100.0%	32.1 / NA	12	D147D (GAT→GAC)	bglB	6‑phospho‑beta‑glucosidase BglB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/6);  total (6/6)

CGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCAT  >  NZ_CP009273/3896534‑3896670                                                                                        |                                                  cGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTc                                                 <  2:86072/90‑1 (MQ=255)     aTGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATg                                             >  1:152986/1‑90 (MQ=255)          gATTTCATTAAACGTCAGCCATAATGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGc                                        <  2:63776/90‑1 (MQ=255)           aTTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCt                                       <  1:64746/90‑1 (MQ=255)                                     cACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGttttt             >  2:181759/1‑90 (MQ=255)                                           ttGTTGGTAGCGAGAAAAGCCCGGGGGGGCGTAATGCTCGAAGTGGTCGATGCCCGCTCGATTACCCCAACCGCCGTAGTTTTTCACCAg       <  2:128820/89‑1 (MQ=255)                                             tttGGTAGCGAGAAAAGCCCGTGCGGGGGTAATGCTCGAAGTGGTCGATGCCCGCTGGATTAGCCCAACCGCCGTAGTTTTTCACCAGcc     <  2:135209/89‑1 (MQ=255)                                                ggcaGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCat  >  1:317190/4‑90 (MQ=255)                                                ggTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCat  >  1:128820/1‑90 (MQ=255)                                                ggTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCat  >  2:294885/1‑90 (MQ=255)                                                                 gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGc                                        <  1:48757/35‑1 (MQ=38)                                                                 gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGc                                        >  2:48757/1‑35 (MQ=38)                                                                                        |                                                  CGACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCAT  >  NZ_CP009273/3896534‑3896670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑