Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	674,442	T→G	T141P (ACT→CCT)	ybeT ←	tetratricopeptide repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	674,442	0	T	G	100.0%	24.6 / NA	9	T141P (ACT→CCT)	ybeT	tetratricopeptide repeat protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/3);  total (6/3)

GTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCC  >  NZ_CP009273/674355‑674519                                                                                        |                                                                              gttgttTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGgat                                                                             <  2:62181/90‑1 (MQ=255)         gCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagca                                                                     >  1:248579/1‑90 (MQ=255)           ggTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagcatg                                                                   <  1:303249/90‑1 (MQ=255)                          tttttGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTc                                                    >  1:298894/1‑90 (MQ=255)                                                          cTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagcatg                                                                   >  1:258003/1‑43 (MQ=255)                                                          cTGAGCCATTTCTGTAGATCAAACCTAAAGGATATtgagcatg                                                                   <  2:258003/43‑1 (MQ=255)                                                           tGAGCCATTTCTGTAGATCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACg                   >  2:152330/1‑90 (MQ=255)                                                            gAGCCATTTCTGTAGATCAAACCTAAAGGATATTgagcatgagcat                                                              <  1:308929/46‑1 (MQ=255)                                                            gAGCCATTTCTGTAGATCAAACCTAAAGGATATTgagcatgagcat                                                              >  2:308929/1‑46 (MQ=255)                                                                            tCAAACCTAAAGGATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGAccc  >  1:230931/1‑90 (MQ=255)                                                                                        |                                                                              GTTGTTTGGCGGTCAGTTTTAACCATTTTTGAGATTCATAATGGTTAACATTAATACCTGAGCCATTTCTGTAGATCAAACCTAAAGTATATTGAGCATGAGCATACCCTTGTTCAGCTGCTTCCTTATACCAGAGAATTGCTTTACGGGTGTCGGGTTTGACCC  >  NZ_CP009273/674355‑674519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑