Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	50,280	T→C	F153S (TTT→TCT)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	50,280	0	T	C	100.0%	41.8 / NA	14	F153S (TTT→TCT)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/9);  total (5/9)

CTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTA  >  NZ_CP009273/50218‑50366                                                               |                                                                                       cTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTg                                                             >  2:20559/1‑90 (MQ=255)      aaTCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAg                                                        <  2:24438/90‑1 (MQ=255)      aaTCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAg                                                        <  2:24439/90‑1 (MQ=255)                                        gcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAgcggc                                                                       >  1:26222/1‑41 (MQ=255)                                        gcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAgcggc                                                                       <  2:26222/41‑1 (MQ=255)                                        gcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTTTATAAAATTTTCGGCTAACGCCCGGTGGGGCGCCGGGcg                      >  1:85063/1‑90 (MQ=255)                                             aCTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTTTTTAGAATTTTCGGCTAACGCCCGGTGCGACGCCCGTCGCGGCt                 >  1:236449/1‑90 (MQ=255)                                                     aGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGc         <  2:167042/90‑1 (MQ=255)                                                     aGCTATTGCTCTGAGATTCAGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGCCTTATCCGGc         <  1:233483/90‑1 (MQ=255)                                                      gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc        <  1:232340/90‑1 (MQ=255)                                                      gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc        <  1:99452/90‑1 (MQ=255)                                                      gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc        <  2:247047/90‑1 (MQ=255)                                                        tATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTACGGCTAGCGCCGGGTGCGACGCCGGGCGCGGCTTTTTCGGCCtt      >  2:232340/1‑90 (MQ=255)                                                            gCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCta  <  2:164494/90‑1 (MQ=255)                                                               |                                                                                       CTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTA  >  NZ_CP009273/50218‑50366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑