Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,503,965	T→C	D67D (GAT→GAC)	hicB →	type II toxin‑antitoxin system antitoxin HicB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,503,965	0	T	C	100.0%	38.7 / NA	13	D67D (GAT→GAC)	hicB	type II toxin‑antitoxin system antitoxin HicB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/8);  total (5/8)

CTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAG  >  NZ_CP009273/1503889‑1504047                                                                             |                                                                                   cTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGTGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAg                                                                       <  1:63185/90‑1 (MQ=255)  tttACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGt                                                                      <  1:209189/90‑1 (MQ=255)               tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc                                                         <  1:271897/90‑1 (MQ=255)               tttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTc                                                         <  2:194929/90‑1 (MQ=255)                ttGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCg                                                        <  2:176738/90‑1 (MQ=255)                                           ttATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCtt                             >  2:200039/1‑90 (MQ=255)                                           ttATCCCTTTACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCtt                             >  2:39318/1‑90 (MQ=255)                                                   ttACCTTCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAg                     <  1:171498/90‑1 (MQ=255)                                                         tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTAc                       >  1:146822/1‑82 (MQ=255)                                                         tCGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTAc                       <  2:146822/82‑1 (MQ=255)                                                          cGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGcc                                                      >  1:327226/1‑50 (MQ=255)                                                          cGCCATTAAATAGTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGcc                                                      <  2:327226/50‑1 (MQ=255)                                                                      gTCACGACCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAag  >  1:236713/1‑90 (MQ=255)                                                                             |                                                                                   CTTTACTGACCGCATTTGATTTTTATTTTGAAGATAACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTATTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAG  >  NZ_CP009273/1503889‑1504047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑