Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,558,439	A→G	Y518H (TAT→CAT)	dosP ←	oxygen‑sensing cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,558,439	0	A	G	100.0%	24.9 / NA	9	Y518H (TAT→CAT)	dosP	oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/6);  total (3/6)

CTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGT  >  NZ_CP009273/1558358‑1558517                                                                                  |                                                                               caaaacccaaacccTTTTTTCCCTTTTCTTTCACCGCGGGGCTAAAAACCTCCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattg                                                                        <  1:199188/88‑1 (MQ=255)      aCCAACCCCTTTTTTACCATTTCGTCCATCGCCGGGGGAAAAAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattgcattg                                                                   <  2:199829/90‑1 (MQ=255)                        aTTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAg                                                 >  2:94656/1‑90 (MQ=255)                                cATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgcg                                         <  1:124588/90‑1 (MQ=255)                                                  cTGCCAGCGGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGCAATCGCGGTTTTTACCCAGGTCGTa                       >  1:239939/1‑90 (MQ=255)                                                      cAGCCTTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTg                   <  2:214898/90‑1 (MQ=255)                                                            ttACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGttt                                     >  1:341828/1‑66 (MQ=255)                                                            ttACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGttt                                     <  2:341828/66‑1 (MQ=255)                                                                       cTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGt  <  2:199615/90‑1 (MQ=255)                                                                                  |                                                                               CTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGT  >  NZ_CP009273/1558358‑1558517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑