Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,573,157	A→T	I148N (ATC→AAC)	yddA ←	ABC transporter ATP‑binding protein/permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,573,157	0	A	T	100.0%	28.5 / NA	9	I148N (ATC→AAC)	yddA	ABC transporter ATP‑binding protein/permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (4/5);  total (4/5)

TTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGA  >  NZ_CP009273/1573077‑1573232                                                                                 |                                                                            tttCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAaga                                                                    <  1:74505/90‑1 (MQ=255)                    aaaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACg                                                 >  2:44330/1‑90 (MQ=255)                     aaGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGt                                                <  2:34018/90‑1 (MQ=255)                          gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                           >  1:191137/1‑90 (MQ=255)                          gATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTc                                           <  1:46887/90‑1 (MQ=255)                                 aTGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTa                                    >  1:157744/1‑90 (MQ=255)                                         tGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAGGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTAtt                            >  1:45257/1‑90 (MQ=255)                                                  gAAGCCAAATGAAAGACTGAGCGTTTTGCTGTTCAGTAGGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTc                   <  2:45257/90‑1 (MQ=255)                                                                   tGAGCGTTTTGCTGTTCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGa  <  2:99861/90‑1 (MQ=255)                                                                                 |                                                                            TTTCCCAAAGAATAACGGTAAAGGTGATCAGCATGCTAAGTGACTGGATGAAGCCAAATGAAAGACTGAGCGTTTTGCTGATCAGTAAGAGAATGTCTTCAGCGATACGTTGGTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGA  >  NZ_CP009273/1573077‑1573232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑