Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,673,542	A→G	K287E (AAG→GAG)	ydgH →	DUF1471 family protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,673,542	0	A	G	100.0%	23.4 / NA	10	K287E (AAG→GAG)	ydgH	DUF1471 family protein YdgH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/8);  total (2/8)

ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTC  >  NZ_CP009273/1673458‑1673607                                                                                     |                                                                  atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                              <  1:324050/90‑1 (MQ=255) atgGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                              <  2:58461/90‑1 (MQ=255)        cGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTa                                                       <  2:217159/90‑1 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                              <  1:171479/82‑1 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGaaa                                                              >  2:171479/1‑82 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                      <  2:145822/90‑1 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                      >  2:238402/1‑90 (MQ=255)         gTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGGAGAAAGGTGCTaa                                                      <  2:93753/90‑1 (MQ=255)                                           aaCATGCCCAAAGTCTCCTATCAGGTTGCAAACCGGCCCGGGGAGAAAGGTGCAAATTATTCCCCCATCCCCCGCCAGTGGCAGGAACGt                    <  1:162887/90‑1 (MQ=255)                                                             taccAGGTTGCAAAACGGGCCGGGGAAAAGGGTGCAAAGTATTCCCACACCCCCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTc  <  1:238402/87‑1 (MQ=255)                                                                                     |                                                                  ATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGGTTGCGAAACGTGCCGCGAAGAAAGGTGCTAAGTATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAATAACCTGACCGTC  >  NZ_CP009273/1673458‑1673607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑