Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	221,335	T→C	noncoding (1078/1542 nt)	BW25113_RS01010 →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	221,335	0	T	C	100.0%	55.1 / NA	20	noncoding (1078/1542 nt)	BW25113_RS01010	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/8);  total (12/8)

TTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAA  >  NZ_CP009273/221248‑221409                                                                                        |                                                                           ttGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGa                                                                          <  2:90926/90‑1 (MQ=255)  tGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGaa                                                                         >  2:341686/1‑90 (MQ=255)      aTCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGt                                                                     <  1:337001/90‑1 (MQ=255)       tCCACAGAACTTTCCAGAGATGGACTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGtt                                                                    >  2:34060/1‑90 (MQ=255)             gAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTa                                                              <  1:268445/90‑1 (MQ=255)                    ccAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCg                                                       >  1:169639/1‑90 (MQ=255)                            ggATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGt                                                                     >  2:228848/1‑68 (MQ=255)                            ggATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGt                                                                     <  1:228848/68‑1 (MQ=255)                              aTTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAgcgc                                             >  1:27419/1‑90 (MQ=255)                              aTTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAgcgc                                             <  2:64227/90‑1 (MQ=255)                                                tgtgAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTttgttg                           >  2:267216/1‑90 (MQ=25)                                                 gtgAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGc                          >  2:110707/1‑90 (MQ=18)                                                 gtgAGACAGGTGCTGCATGGCTGTCGCCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGc                          >  2:53891/1‑90 (MQ=255)                                                     gaCAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGc                      >  2:302808/1‑90 (MQ=18)                                                               gcATGGCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTccggccg            >  2:285403/1‑90 (MQ=18)                                                                    gCTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAAc       >  1:89176/1‑90 (MQ=18)                                                                     cTGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACt      <  2:153841/90‑1 (MQ=25)                                                                      tGTCGTCAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTc     <  2:169639/90‑1 (MQ=25)                                                                         cgtcAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCaaa  <  2:56397/90‑1 (MQ=18)                                                                         cgtcAGCTCGTGTTGCGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCaaa  >  1:131284/1‑90 (MQ=18)                                                                                        |                                                                           TTGACATCCACAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTGTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCTTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAA  >  NZ_CP009273/221248‑221409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑