| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 231,042 | A→C | G76G (GGT→GGG) | gloB ← | hydroxyacylglutathione hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 231,042 | 0 | A | C | 80.0% | 35.7 / 3.0 | 15 | G76G (GGT→GGG) | gloB | hydroxyacylglutathione hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base C (5/7); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGA > NZ_CP009273/230957‑231118 | ttACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCAt < 1:126693/90‑1 (MQ=255) gCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTtgtg > 2:191136/1‑90 (MQ=255) cAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGaaa < 1:14939/90‑1 (MQ=255) gTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGt > 1:24366/1‑90 (MQ=255) tttCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGtt > 1:2423/1‑90 (MQ=255) aTCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTAc < 1:50392/90‑1 (MQ=255) cTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTAc < 1:73656/88‑1 (MQ=255) cTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTAc > 2:73656/1‑88 (MQ=255) ccTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGt < 2:2423/90‑1 (MQ=255) tgttgtTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATc < 1:242751/90‑1 (MQ=255) tgttgtTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATc < 1:59170/90‑1 (MQ=255) ttCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgtggt > 2:61341/1‑90 (MQ=255) tCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgtggtg > 1:216376/1‑90 (MQ=255) tATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGAtggt < 1:287680/90‑1 (MQ=255) gtgtCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTga > 1:55919/1‑90 (MQ=255) | TTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGA > NZ_CP009273/230957‑231118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |