Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,084,6530AG66.7% 14.3 / 18.6 32G358G (GGT→GGC) frvBPTS fructose‑like transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (6/3);  new base G (9/9);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGC  >  NZ_CP009273/4084569‑4084735
                                                                                    |                                                                                  
gACTGTGGCCCCGACCACAATGGCACTACTGGCCGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGACCGTCGCAAGGCccgc                                                                               <  2:801762/90‑1 (MQ=255)
     gggCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGGAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGa                                                                          >  2:122250/2‑90 (MQ=255)
      ggCACCGACCACAATGGCACTACTGGCAGCCTTTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGCCCCGCCGCGAc                                                                         <  1:1004219/90‑1 (MQ=255)
          ccGCCCACAGGGGCACTCCTGGCACCCTTTTTTCCCTGGGGGGAAAACAGTTTGGGCGCAATGACCGTCGAAAGCCCCGCCGCGACGggc                                                                     <  2:119448/90‑1 (MQ=255)
          ccGCCCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggc                                                                     <  1:980685/90‑1 (MQ=255)
            gACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGggcgg                                                                   >  1:196507/1‑90 (MQ=255)
            gACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGggcgg                                                                   >  1:178224/1‑90 (MQ=255)
              ccACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCa                                                                 >  2:283184/1‑90 (MQ=255)
                           actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                    >  2:606767/1‑90 (MQ=255)
                           actGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAAc                                                    >  2:1126956/1‑90 (MQ=255)
                            ctGGCAGCTTTTTTTTCCTGTGGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACg                                                   <  1:964349/90‑1 (MQ=255)
                                 aGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCcgac                                              <  2:661263/90‑1 (MQ=255)
                                    ctttttttCGTCTGGGGAAAACAGTTTGGGCCAAAGAAACGCGGGAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGAt                                           <  2:863611/87‑1 (MQ=255)
                                    cttttttcCCGGGCGGGTAAACAGTTGGGCCGCAAGGAACGCCGAAAGCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGAt                                           <  2:395543/87‑1 (MQ=255)
                                    ctctTTTCCCTGGCGGGTAAACAGTTGGGGCGAAATGACCGCCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGAt                                           <  2:1075122/90‑1 (MQ=255)
                                           tCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAc                                    <  1:733355/90‑1 (MQ=255)
                                           tCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAc                                    <  2:196507/90‑1 (MQ=255)
                                           tCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAc                                    <  1:501961/90‑1 (MQ=255)
                                           tCCTGGCGGGAAAACGTTTTGGGCGAAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAc                                    <  1:1070501/90‑1 (MQ=255)
                                           cccTGCCGGGTAAACAGTTGGGGCGCATCGCCCGCGGCAGGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAc                                    <  2:732618/89‑1 (MQ=255)
                                             cTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCa                                  >  2:684716/1‑90 (MQ=255)
                                              tGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAg                                 >  2:45127/1‑90 (MQ=255)
                                              tGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAg                                 >  1:401746/1‑90 (MQ=255)
                                              tGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAcc                                   <  1:354375/88‑1 (MQ=255)
                                              tGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTAcc                                   >  2:354375/1‑88 (MQ=255)
                                               gTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGt                                >  1:732618/1‑90 (MQ=255)
                                                tCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTc                               >  1:460533/1‑90 (MQ=255)
                                                                     aaTGACCGTCGCAAGCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagcag          <  2:216150/90‑1 (MQ=255)
                                                                     aaTGAACGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagcag          >  1:677624/1‑90 (MQ=255)
                                                                     aaTGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagcag          >  2:1089727/1‑90 (MQ=255)
                                                                           cGTCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGaa    <  1:45127/90‑1 (MQ=255)
                                                                             tCGCAAGGCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGc  >  2:74755/1‑90 (MQ=255)
                                                                                    |                                                                                  
GACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAACGTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGC  >  NZ_CP009273/4084569‑4084735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑