| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,531,275 | G→A | L98L (CTG→TTG) | narW ← | nitrate reductase molybdenum cofactor assembly chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,531,275 | 0 | G | A | 76.3% | 74.4 / 19.4 | 38 | L98L (CTG→TTG) | narW | nitrate reductase molybdenum cofactor assembly chaperone |
| Reads supporting (aligned to +/- strand): ref base G (4/5); new base A (18/11); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
ATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAAC > NZ_CP009273/1531191‑1531360 | aTCCTTCTTTCGCCTGATGGTCAAGCAGTACACTTAAATACACCAGATACAGCGGTAGGTAATCGGGCAGTTTGCGAGAATTCAAGTGCa > 2:221849/1‑90 (MQ=255)aTCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCa > 1:233071/1‑90 (MQ=255)aTCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCa > 1:40674/1‑90 (MQ=255) cttcttTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGc < 2:1033231/90‑1 (MQ=255) cttTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGa < 1:127980/90‑1 (MQ=255) tttCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAc < 2:574354/90‑1 (MQ=255) cGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCtt > 1:767088/1‑90 (MQ=255) gCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCttt > 2:865789/1‑90 (MQ=255) ccTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCtttt > 2:136739/1‑90 (MQ=255) gATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCAt > 1:627023/1‑90 (MQ=255) aGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCcagc < 2:429327/90‑1 (MQ=255) aGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCcagc < 1:865789/90‑1 (MQ=255) aGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCcagc > 2:1050049/1‑90 (MQ=255) gTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCcagca > 1:933217/1‑90 (MQ=255) gTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCcagca < 1:1050049/90‑1 (MQ=255) cTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGc > 1:395006/1‑90 (MQ=255) ccAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCt > 2:91025/1‑90 (MQ=255) ccAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCt > 1:454715/1‑90 (MQ=255) ccAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCt > 2:173852/1‑90 (MQ=255) aGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTgg > 1:966375/1‑90 (MQ=255) gTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAc < 2:1092983/43‑1 (MQ=255) gTAGATAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGAc > 1:1092983/1‑43 (MQ=255) tAATCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGAc > 2:314347/1‑90 (MQ=255) aaTCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACt < 1:711409/90‑1 (MQ=255) aTCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTc > 2:746191/1‑90 (MQ=255) aTCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTc < 1:55579/90‑1 (MQ=255) aTCGGGCAGTTCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTc > 1:296150/1‑90 (MQ=255) gggCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCcagca > 1:514879/1‑53 (MQ=255) gggCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCcagca < 2:514879/53‑1 (MQ=255) ttCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAAc < 2:966375/90‑1 (MQ=255) ttCCCGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAAc < 2:556057/90‑1 (MQ=255) tccccGACAATCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAAc < 2:40674/88‑1 (MQ=255) gACAATCCAACTGCAGGCCGCCCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGtt < 2:1017412/90‑1 (MQ=255) aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa > 2:650988/1‑90 (MQ=255) aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa > 2:818641/1‑90 (MQ=255) aTCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGaa > 1:114022/1‑90 (MQ=255) tCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAAc < 1:1086952/90‑1 (MQ=255) tCCAACTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAAc < 2:767088/90‑1 (MQ=255) | ATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGGCCGACCTTTTCATACTCCGCCAGCAGGTCCACCATTGCCTGGCCGCGATCGCGGGACTCGGCATGAACATGTTCGAAC > NZ_CP009273/1531191‑1531360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |