| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,380,341 | G→A | intergenic (+31/‑72) | yfbL → / → yfbM | M28 family metallopeptidase/YfbM family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,341 | 0 | G | A | 75.0% | 11.8 / 6.1 | 16 | intergenic (+31/‑72) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base G (0/4); new base A (12/0); total (12/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-03 | |||||||||||
GTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAA > NZ_CP009273/2380272‑2380419 | gTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACAAGCACattttttttt > 1:467095/1‑90 (MQ=255) tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATc < 1:536517/90‑1 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCAGAAAAAACAACAAGCACAATTTATTTTTTCTCCTCtttttttat > 2:304761/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCAGAAAAAAAGAAAAGAAAAATTTATTTTTTCTCCTCtttttttaa > 2:1068455/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAATACAAGTACTTTTTATTTTTCCCCATCACtttttaa > 1:292137/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGACAAGCACAATTTTTTTTTCCCCCTCTGtttttaa > 1:681854/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCAAAAAAAAGAAAAGCACAATTTTTTTTTCCTCCTCTTTTTTaaa > 1:822339/1‑89 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGACAAGCACATTTTTTTTTTTCTCCTCtttttttat > 1:946798/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGCACATTTTTTTTTTTCTCATCTGtttttaa > 2:656584/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGAAAAGCACAATTTTTTTTTTCTCTTCTGtttttaa > 2:173481/1‑87 (MQ=255) acaGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGACAAGCACAATTTTTTTTTCCCCATTTTTTTTTAAATGCGgaaaaa > 1:1030920/1‑87 (MQ=255) acaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACAt < 2:56293/90‑1 (MQ=255) gTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACATGCACATTTTTTTTTTCCTCCTCTGTTTTTAAATGGGAAAAAAGaa > 1:231106/1‑90 (MQ=255) ataaAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACATGCACATTTTATTTTTCCCCATCTGTTTTTAAATGCGAAAAAAGAAAATCAAc > 1:56293/1‑90 (MQ=255) ggCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaataaat < 1:304761/90‑1 (MQ=255) gTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGaa < 2:681854/90‑1 (MQ=255) | GTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAA > NZ_CP009273/2380272‑2380419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |