Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,038,183	(T)5→4	intergenic (+78/‑236)	BW25113_RS10330 → / → yeeJ	tRNA‑Asn/inverse autotransporter adhesin YeeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,038,179	0	T	.	100.0%	143.4 / NA	34	intergenic (+74/‑240)	BW25113_RS10330/yeeJ	tRNA‑Asn/inverse autotransporter adhesin YeeJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/18);  total (16/18)

CAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCG  >  NZ_CP009273/2038095‑2038255                                                                                     |                                                                             cAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGttttaa                                                                         <  1:23313/90‑3 (MQ=255)  agagGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGttttaac                                                                        <  1:625252/90‑4 (MQ=255)           aaaTTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAgc                                                              <  2:1093819/90‑1 (MQ=255)           aaaTTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAgc                                                              <  1:873749/90‑1 (MQ=255)            aaTTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAgcg                                                             <  2:495429/90‑1 (MQ=255)                  aaaaaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCg                                                       <  1:895533/90‑1 (MQ=255)                  aaaaaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCg                                                       <  2:473653/90‑1 (MQ=255)                  aaaaaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCg                                                       <  2:698905/90‑1 (MQ=255)                     aaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGAcc                                                    <  1:529566/90‑1 (MQ=255)                     aaTTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGAcc                                                    <  2:1069583/90‑1 (MQ=255)                                        aTGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGttt                                 <  1:568794/90‑1 (MQ=255)                                        aTGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGttt                                 >  2:713250/1‑90 (MQ=255)                                            cACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAgc                                                              >  2:700003/1‑57 (MQ=255)                                            cACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAgc                                                              <  1:700003/57‑1 (MQ=255)                                                gACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGa                         >  1:1512146/1‑90 (MQ=255)                                                gACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGa                         <  1:1471299/90‑1 (MQ=255)                                                gACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGa                         >  2:740940/1‑90 (MQ=255)                                                gACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGa                         >  2:8818/1‑90 (MQ=255)                                                  ccTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAAc                       >  2:1001000/1‑90 (MQ=255)                                                  ccTTAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAAc                       >  2:826305/1‑90 (MQ=255)                                                    ttAGTTGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGc                     >  1:706000/1‑90 (MQ=255)                                                         tGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTGTGAACGCCATCa                <  1:458979/90‑1 (MQ=255)                                                         tGAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCa                <  1:8818/90‑1 (MQ=255)                                                          gAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGAcc                                                    >  2:1007022/1‑53 (MQ=255)                                                          gAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGAcc                                                    <  1:1007022/53‑1 (MQ=255)                                                          gAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCAc               >  2:1538459/1‑90 (MQ=255)                                                          gAACATTGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCAc               >  2:71638/1‑90 (MQ=255)                                                               ttGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCAttt          >  2:213196/1‑90 (MQ=255)                                                               ttGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCAttt          >  1:208280/1‑90 (MQ=255)                                                               ttGTTTTTTAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCAttt          >  1:1354077/1‑90 (MQ=255)                                                                    ttttAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTcccc     >  1:1456634/1‑90 (MQ=255)                                                                      ttAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTc   <  2:1456634/90‑1 (MQ=255)                                                                       tAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCg  <  2:1258361/90‑1 (MQ=255)                                                                       tAACGGATAGCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCg  <  1:1313174/90‑1 (MQ=255)                                                                                gCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTcccc     >  2:1411514/1‑78 (MQ=255)                                                                                gCGGG‑TTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTcccc     <  1:1411514/78‑1 (MQ=255)                                                                                     |                                                                             CAGAGGAGCCAAATTCTAAAAATTCGCTTTTTTAGCGCAATGTCACTGACCTTAGTTGAACATTGTTTTTTAACGGATAGCGGGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCG  >  NZ_CP009273/2038095‑2038255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑