| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 264,689 | (A)5→4 | intergenic (‑18/+311) | ykfA ← / ← perR | GTPase family protein/LysR substrate‑binding domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 264,685 | 0 | A | . | 95.2% | 157.8 / 3.7 | 42 | intergenic (‑14/+315) | ykfA/perR | GTPase family protein/LysR substrate‑binding domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base . (16/24); total (18/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTT > NZ_CP009273/264596‑264769 | gAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGa > 2:1454143/1‑90 (MQ=255) ctctGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGaaaataat > 2:584627/1‑86 (MQ=255) gATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATaaaa > 2:676599/1‑90 (MQ=255) aTACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATaaaaa < 2:934703/90‑1 (MQ=255) tACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATaaaaaa > 1:1363888/1‑90 (MQ=255) cATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACgg > 2:830640/1‑90 (MQ=255) ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTac < 2:1573962/90‑2 (MQ=255) ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAg > 1:811677/1‑90 (MQ=255) ttGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAg > 2:1528499/1‑90 (MQ=255) gCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGaa > 1:1247529/1‑90 (MQ=255) gCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTc < 2:756396/90‑1 (MQ=255) gCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTc < 2:88683/90‑1 (MQ=255) gCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTc > 2:213966/1‑90 (MQ=255) aTTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGtt < 1:259864/90‑1 (MQ=255) ttGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTa > 2:1046389/1‑90 (MQ=255) cTGAAACGACTTCAAACCTTCAGAATTATTCATCACTTCTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAAtt < 1:751861/90‑1 (MQ=255) cTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAAtt < 2:581613/90‑1 (MQ=255) cTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAAtt < 2:811677/90‑1 (MQ=255) aaCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt < 2:1247529/90‑1 (MQ=255) aaCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt < 2:126077/90‑1 (MQ=255) aaCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt < 2:113941/90‑1 (MQ=255) aaCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt < 1:1127617/90‑1 (MQ=255) aCGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTGCGCCGTTAATTGCGTa < 2:1134097/90‑1 (MQ=255) ttCAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTc < 1:1399946/90‑1 (MQ=255) cAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCag < 1:434567/90‑1 (MQ=255) cAAACCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCag < 1:1428152/90‑1 (MQ=255) aaCCTTCAGAATTATTCATCACTCCTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCagag < 1:1163366/90‑1 (MQ=255) aaCCTTCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCagag < 2:1524034/90‑1 (MQ=255) ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt > 2:1301322/1‑76 (MQ=255) ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGt < 1:1301322/76‑1 (MQ=255) ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat < 2:1130005/90‑1 (MQ=255) ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat < 1:1046389/90‑1 (MQ=255) ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat < 2:1081707/90‑1 (MQ=255) ttCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgat < 1:1568893/90‑1 (MQ=255) tCAGAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGtgatg > 1:1066482/1‑90 (MQ=255) gAATTATTCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGata > 2:77739/1‑90 (MQ=255) tattCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGatatata > 1:1018140/1‑90 (MQ=255) tattCATCACTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGatatata > 1:277302/1‑90 (MQ=255) atcaCTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGa < 2:1399865/90‑1 (MQ=255) tcaCTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGaa > 2:774471/1‑90 (MQ=255) tcaCTACTCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGaa > 2:1367131/1‑90 (MQ=255) actCCTCTGAAAAATAATAAAAAGCGGTAGGATCGTGAGATTCCGCCGTAAATTGCGGATGTTCAGAGGGATGAGATATATCTGAAGAtt > 2:204600/1‑90 (MQ=25) actCCTCTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGAttt > 1:846822/1‑90 (MQ=255) cctcTG‑AAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGAtttttt > 2:1399261/1‑90 (MQ=255) | GAAGCCGCTCTGATACCCATTGCGGCAGATCAGCAAGCGATTGCTGAAACGACTTCAAACCTTCAGAATTATTCATCACTACTCCTCTGAAAAATAATAAAAAACGGTAGAATCGTGAGATTCCGCCGTTAATTGCGTATGTTCAGAGTGATGATATATATCTGAAGATTTTTT > NZ_CP009273/264596‑264769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |