Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,103,871 (A)7→6 intergenic (+71/+78) BW25113_RS15410 → / ← yghD tRNA‑Phe/GspM family type II secretion system protein YghD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,103,8650A.97.9% 194.8 / ‑3.3 48intergenic (+65/+84)BW25113_RS15410/yghDtRNA‑Phe/GspM family type II secretion system protein YghD
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (17/30);  total (18/30)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCC  >  NZ_CP009273/3103776‑3103954
                                                                                         |                                                                                         
ggTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGa                                                                                           <  2:1403966/90‑1 (MQ=255)
 gTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaa                                                                                          <  1:904055/90‑1 (MQ=255)
   tAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaaa                                                                                        >  1:253689/1‑90 (MQ=255)
     gATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaaaaa                                                                                      >  1:50215/1‑90 (MQ=255)
      aTTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaaaaat                                                                                     <  2:719666/90‑2 (MQ=255)
      aTTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaaaaaat                                                                                     <  1:592359/90‑2 (MQ=255)
          cGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGc                                                                                <  2:597176/90‑1 (MQ=255)
           gAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCt                                                                               >  1:1385115/1‑90 (MQ=255)
           gAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCt                                                                               >  2:1581692/1‑90 (MQ=255)
           gAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCt                                                                               >  2:565258/1‑90 (MQ=255)
               ccGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTtata                                                                           <  1:446001/90‑1 (MQ=255)
               ccGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTtata                                                                           >  2:130924/1‑90 (MQ=255)
                    caccacTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTc                                                                      <  2:1150715/90‑1 (MQ=255)
                    caccacTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTc                                                                      <  1:1225938/90‑1 (MQ=255)
                       cacTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTgg                                                                   <  1:11213/90‑1 (MQ=255)
                       cacTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTgg                                                                   >  2:244223/1‑90 (MQ=255)
                        acTAATTCTTAAGAACCTGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTggg                                                                  <  1:640911/90‑1 (MQ=255)
                        acTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTggg                                                                  <  1:1301192/90‑1 (MQ=255)
                        acTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTggg                                                                  <  1:988367/90‑1 (MQ=255)
                        acTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTggg                                                                  <  2:512116/90‑1 (MQ=255)
                                ttAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTAc                                                          >  2:1178386/1‑90 (MQ=255)
                                ttAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGGCTGGGGGAATTAc                                                          >  1:860635/1‑90 (MQ=255)
                                      aCCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGc                                                    <  2:1395498/90‑1 (MQ=255)
                                      aCCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGc                                                    <  1:1581692/90‑1 (MQ=255)
                                      aCCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGc                                                    <  2:167618/90‑1 (MQ=255)
                                      aCCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGc                                                    <  1:587685/90‑1 (MQ=255)
                                      aCCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGc                                                    <  2:267142/90‑1 (MQ=255)
                                      aCCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGc                                                    <  1:130924/90‑1 (MQ=255)
                                        ccGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCa                                                  <  1:1057272/90‑1 (MQ=255)
                                        ccGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCa                                                  <  1:1178386/90‑1 (MQ=255)
                                         cGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTGGCTTATAAAGTCTGGGGGAATTACTCTCGCCAc                                                 >  2:1195365/1‑90 (MQ=255)
                                         cGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCAc                                                 >  2:218769/1‑90 (MQ=255)
                                               caAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAAc                                           <  2:939133/90‑1 (MQ=255)
                                                   gCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACgaga                                       <  2:381671/90‑1 (MQ=255)
                                                     gggTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGt                                     >  2:693056/1‑90 (MQ=255)
                                                     gggTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGt                                     >  1:1379485/1‑90 (MQ=255)
                                                           tttcTTTTGGGTTTGACAAAAAACTTCACGAAAAAAATAGGTTTTAAAGTTTGGGGGGATTTCTCTCCCCCCGTTAACGAGAGGAAtttt                               >  1:912329/4‑90 (MQ=255)
                                                              cTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTAtt                            <  1:613272/90‑1 (MQ=255)
                                                              cTTTTGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTAtt                            <  2:1010989/90‑1 (MQ=255)
                                                                tttGGATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGa                          >  1:1157390/1‑90 (MQ=255)
                                                                   ggATCTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGatat                       <  1:100741/90‑1 (MQ=255)
                                                                       cTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAAt                   <  1:218769/90‑1 (MQ=255)
                                                                       cTGACAATAACCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAAt                   <  1:1484794/90‑1 (MQ=255)
                                                                               aaCCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGAt           <  1:883221/90‑1 (MQ=255)
                                                                               aaCCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGAt           <  2:1003582/90‑1 (MQ=255)
                                                                               aaCCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGAt           <  1:565258/90‑1 (MQ=255)
                                                                               aaCCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGAt           <  1:231189/90‑1 (MQ=255)
                                                                               aaCCTTCACGAAAAAATTAGCTTATAAAGTCTGGGGGAAATACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGAt           <  1:188810/90‑1 (MQ=255)
                                                                                   ttCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTg             <  1:452550/84‑1 (MQ=255)
                                                                                   ttCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTg             >  2:452550/1‑84 (MQ=255)
                                                                                   ttCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACtt       >  1:1293275/1‑90 (MQ=255)
                                                                                   ttCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACtt       >  2:1427811/1‑90 (MQ=255)
                                                                                   ttCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACtt       >  1:324655/1‑90 (MQ=255)
                                                                                    tCACGAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACttt      >  2:685820/1‑90 (MQ=255)
                                                                                         aaaaaaaTTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTAccc  >  1:1545671/1‑90 (MQ=255)
                                                                                         |                                                                                         
GGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCC  >  NZ_CP009273/3103776‑3103954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: