| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,240,670 | Δ1 bp | coding (55/441 nt) | BW25113_RS06225 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,240,667 | 0 | G | . | 94.1% | 60.3 / ‑1.0 | 17 | coding (52/441 nt) | BW25113_RS06225 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base . (7/9); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
ATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAA > NZ_CP009273/1240582‑1240744 | aTCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATgggcg > 1:714216/1‑88 (MQ=255) cTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGAt < 2:988441/90‑1 (MQ=255) cTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGAt < 2:1609/90‑1 (MQ=255) gttgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTg < 1:1182857/90‑1 (MQ=255) tgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTgcg < 1:548185/90‑1 (MQ=255) ttttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTTGCt > 2:1459171/1‑84 (MQ=255) ttttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCt < 1:1459171/84‑1 (MQ=255) tGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTAtt < 1:451992/90‑1 (MQ=255) tgtgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGaa < 2:554648/90‑1 (MQ=255) tgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGGTTTTGCTGCGCTTATTTaaa > 1:1434637/1‑89 (MQ=255) aaaaTAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAAt < 1:1451251/90‑1 (MQ=255) atTTTAGACAGTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGGTTTTGCTGCGCTTTTTTAAAATTTAGAATTGATTAATc > 1:1177363/1‑90 (MQ=255) gTAGCCAGTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTTTTTAAAATTTAGAATTGATTAATCCTTCTGGCAg > 1:366036/1‑90 (MQ=255) gTCAGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTTGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTTGCAGATTAAGc > 2:1457795/1‑90 (MQ=255) aGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGGTGCGCCTTTTTAAAAATTAGAATTGATTAAACCTTTTGGCAGATTTAGCCaa > 1:1250716/1‑90 (MQ=255) aGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTTAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCaa > 1:1379425/1‑90 (MQ=255) aGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGaa < 1:97416/51‑1 (MQ=255) aGGCGTTAAAAAT‑GGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGaa > 2:97416/1‑51 (MQ=255) | ATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTGGCTGCGCTTATTGAACATTTAGAATTGATCAATCCTTCTGGCAGATTAAGCCAA > NZ_CP009273/1240582‑1240744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |