Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,632,437	(T)7→6	intergenic (‑71/+8)	BW25113_RS08135 ← / ← BW25113_RS25620	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,632,431	0	T	.	100.0%	176.9 / NA	42	intergenic (‑65/+14)	BW25113_RS08135/BW25113_RS25620	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/23);  total (19/23)

TTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTT  >  NZ_CP009273/1632343‑1632507                                                                                         |                                                                             ttCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGAtt                                                                             >  1:1347091/1‑90 (MQ=255)  tCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGAttt                                                                            >  1:200094/1‑90 (MQ=255)           ggATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCatgat                                                                  <  1:851199/90‑1 (MQ=255)           ggATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCatgat                                                                  <  2:50505/90‑1 (MQ=255)           ggATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACAACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCatgat                                                                  <  2:568349/90‑1 (MQ=255)             atatTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGt                                                                <  1:1102516/90‑1 (MQ=255)               atTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTa                                                              >  1:15790/1‑90 (MQ=255)               atTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTa                                                              >  1:503581/1‑90 (MQ=255)                 tCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTAcc                                                            <  2:128923/90‑1 (MQ=255)                   gcGCATGATGGTGTTAACTCCAGTCATCGCTGGCCCTGCCCCTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  1:878474/89‑1 (MQ=255)                   aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGGTGTTACCgt                                                          <  2:154188/90‑1 (MQ=255)                   aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  2:572494/90‑1 (MQ=255)                   aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  2:559010/90‑1 (MQ=255)                   aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  1:1340325/90‑1 (MQ=255)                   aCGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  2:497154/90‑1 (MQ=255)                   aCGCACGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  2:452599/90‑1 (MQ=255)                        tgatgGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTAt                                                     <  2:943823/90‑1 (MQ=255)                         gatgGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTAtg                                                    >  2:286574/1‑90 (MQ=255)                           tgGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTAtgtg                                                  <  2:880253/90‑1 (MQ=255)                            ggTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGa                                                 <  1:196421/90‑1 (MQ=255)                                  aaCTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATcc                                           >  1:101232/1‑90 (MQ=255)                                    cTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCAt                                         >  2:1251297/1‑90 (MQ=255)                                    cTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCAt                                         >  1:1441078/1‑90 (MQ=255)                                    cTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCAt                                         >  1:1472270/1‑90 (MQ=255)                                    cTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCAt                                         >  2:1486871/1‑90 (MQ=255)                                     tCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATc                                        <  1:342618/90‑1 (MQ=255)                                     tCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATc                                        <  1:81512/90‑1 (MQ=255)                                     tCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATc                                        <  2:227366/90‑1 (MQ=255)                                       cAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          >  1:60073/1‑70 (MQ=255)                                       cAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCgt                                                          <  2:60073/70‑1 (MQ=255)                                       cAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGa                                      >  1:297436/1‑90 (MQ=255)                                       cAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGa                                      >  2:930454/1‑90 (MQ=255)                                       cAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGa                                      >  2:605917/1‑90 (MQ=255)                                       cAGTCATCGCTGACACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑‑TTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGaa                                     >  1:402682/1‑90 (MQ=255)                                       cAGTCATCGCTGACACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑‑TTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGaa                                     >  1:402685/1‑90 (MQ=255)                                          tCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATa                                   <  1:775496/90‑1 (MQ=255)                                                           aCTGCTGGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAg                  <  2:720700/90‑1 (MQ=255)                                                            ctgctgGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGc                 >  2:1543003/1‑90 (MQ=255)                                                              gctgGTTCAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTc               >  2:63732/1‑90 (MQ=255)                                                                     cAGAGTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAgg        <  2:1347091/90‑1 (MQ=255)                                                                      agagTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGa       >  2:615252/1‑90 (MQ=255)                                                                      agagTTAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGa       >  2:517957/1‑90 (MQ=255)                                                                          ttAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCt   <  1:859061/90‑1 (MQ=255)                                                                           tAAAAAAACTATGA‑TTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCtt  >  2:1370615/1‑90 (MQ=255)                                                                                         |                                                                             TTCGCCATTTGGATATTCACGCATGATGGTGTTAACTCCAGTCATCGCTGGCACTACCACTGCTGGTTCAGAGTTAAAAAAACTATGATTTTTTTCATGATGTTACCGTAGTATGTGAGTATCCATCGAATAGACACCAAGCAAAAAAGCTCCCGAAGGAGCCTT  >  NZ_CP009273/1632343‑1632507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑