| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,736 | A→T | intergenic (+105/‑87) | kefC → / → folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,736 | 0 | A | T | 95.7% | 172.3 / ‑0.7 | 47 | intergenic (+105/‑87) | kefC/folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base T (26/19); total (28/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01 | |||||||||||
CACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGG > NZ_CP009273/49651‑49813 | cacTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATtttt < 1:575060/90‑1 (MQ=255)cacTTTACAGCTAACTGTTTGGTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATtttt > 1:14712/1‑90 (MQ=255) ttACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTc < 1:942748/90‑1 (MQ=255) ttACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTc > 2:839259/1‑90 (MQ=255) cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc < 1:1422201/90‑1 (MQ=255) aaCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatcat > 1:765957/1‑90 (MQ=255) aaCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatcat > 1:1563392/1‑90 (MQ=255) aaCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatcat > 1:452698/1‑90 (MQ=255) aaCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc > 2:255967/1‑88 (MQ=255) aaCTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAAcatc < 1:255967/88‑1 (MQ=255) tgtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCa > 2:885057/1‑90 (MQ=255) tttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCAcc < 1:1007363/90‑1 (MQ=255) cATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga < 1:563965/90‑1 (MQ=255) cATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga < 1:110416/90‑1 (MQ=255) aTTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCgacgac < 2:452698/90‑1 (MQ=255) aTTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCgacgac < 2:1563392/90‑1 (MQ=255) aaTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGttt < 1:1093735/90‑1 (MQ=255) aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCCCCAGTCGACGACGGTTTa < 1:1211735/90‑1 (MQ=255) aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTa < 2:565762/90‑1 (MQ=255) gcggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACg > 1:1033971/1‑90 (MQ=255) cggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGc > 1:718311/1‑90 (MQ=255) ggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCt < 2:731005/90‑1 (MQ=255) gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa < 2:149227/90‑1 (MQ=255) gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa > 2:60709/1‑90 (MQ=255) tCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGt < 2:140005/90‑1 (MQ=255) gggAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAg < 2:153241/90‑1 (MQ=255) gagagaGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTg < 1:255059/90‑1 (MQ=255) gagagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga > 1:1031321/1‑65 (MQ=255) gagagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTcgacga < 2:1031321/65‑1 (MQ=255) gagagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGc > 1:1573698/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACg < 2:665495/73‑1 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACg > 1:665495/1‑73 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg > 2:1147036/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg > 2:81160/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg > 2:166818/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg > 1:203595/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg > 1:919665/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg > 2:1183088/1‑90 (MQ=255) gCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACa > 2:397047/1‑90 (MQ=255) gTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGGCGGTTTACGCTTTACGTATAGTGGCGACAAt > 1:674502/1‑90 (MQ=255) gTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAt > 1:796099/1‑90 (MQ=255) gTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAt > 2:89227/1‑90 (MQ=255) ggACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACCACGGTTTACGCTTTACGTATAGTGGCGACAAttt > 2:103144/1‑90 (MQ=255) tCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAttttttt > 2:306506/1‑90 (MQ=255) tCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAttttttt > 2:185663/1‑90 (MQ=255) cGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAtttttttt > 1:1001311/1‑90 (MQ=255) gcagAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCgg > 1:85366/1‑90 (MQ=255) | CACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGG > NZ_CP009273/49651‑49813 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |