Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,803,201	A→G	V97A (GTC→GCC)	yniB ←	YniB family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,803,201	0	A	G	98.0%	159.0 / ‑4.0	51	V97A (GTC→GCC)	yniB	YniB family protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (20/30);  total (20/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGTTATACCAGAATAG  >  NZ_CP009273/1803118‑1803283                                                                                    |                                                                                   gAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCaaaaa                                                                              >  1:752794/1‑90 (MQ=255)   aTGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATc                                                                            <  2:730770/90‑1 (MQ=255)   aTGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATc                                                                            <  2:392724/90‑1 (MQ=255)   aTGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATc                                                                            <  2:219279/90‑1 (MQ=255)     gAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAg                                                                          <  2:413255/90‑1 (MQ=255)         tGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATg                                                                      <  2:832426/90‑1 (MQ=255)         tGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATg                                                                      <  2:528617/90‑1 (MQ=255)           aTCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGaa                                                                    >  1:713508/1‑90 (MQ=255)               tCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATc                                                                >  1:549913/1‑90 (MQ=255)                   cacCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCAccc                                                            <  2:403550/90‑1 (MQ=255)                   cacCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCAccc                                                            <  2:106191/90‑1 (MQ=255)                   cacCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCAccc                                                            <  2:119159/90‑1 (MQ=255)                   cacCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCAccc                                                            <  2:329741/90‑1 (MQ=255)                   cacCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCAccc                                                            <  2:904331/90‑1 (MQ=255)                   cacCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAccc                                                            <  1:1180394/90‑1 (MQ=255)                    acCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCa                                                           <  1:376941/90‑1 (MQ=255)                    acCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCa                                                           <  1:119579/90‑1 (MQ=255)                     ccTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAg                                                          <  2:651475/90‑1 (MQ=255)                       ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGATAATCACCCAGaa                                                        >  1:1065697/1‑90 (MQ=255)                       ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGaa                                                        >  2:37500/1‑90 (MQ=255)                       ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGaa                                                        >  1:869025/1‑90 (MQ=255)                       ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGaa                                                        >  1:261264/1‑90 (MQ=255)                        tCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAAc                                                       >  2:1122219/1‑90 (MQ=255)                               agaagCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGt                                                >  1:1255001/1‑90 (MQ=255)                               agaagCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGt                                                >  1:1268088/1‑90 (MQ=255)                                      cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcc                                         <  1:815424/90‑1 (MQ=255)                                      cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcc                                         <  2:350237/90‑1 (MQ=255)                                      cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcc                                         <  2:1453905/90‑1 (MQ=255)                                      cgcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcc                                         <  2:774655/90‑1 (MQ=255)                                       gcCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca                                        <  2:1160733/90‑1 (MQ=255)                                             cGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTa                                  >  2:1210708/1‑90 (MQ=255)                                                  cATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAAtt                             >  2:495215/1‑90 (MQ=255)                                                  cATCCGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAAtt                             >  2:593001/1‑90 (MQ=255)                                                     ccGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAAt                                                                 >  2:431063/1‑51 (MQ=255)                                                     ccGGGCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAAt                                                                 <  1:431063/51‑1 (MQ=255)                                                       gggCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca                                        <  1:1015126/74‑1 (MQ=255)                                                       gggCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGcca                                        >  2:1015126/1‑74 (MQ=255)                                                         gCACCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTc                      >  2:396437/1‑90 (MQ=255)                                                           aCCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCgg                    <  1:286089/90‑1 (MQ=255)                                                           aCCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCgg                    <  1:593001/90‑1 (MQ=255)                                                           aCCGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCgg                    <  1:166863/90‑1 (MQ=255)                                                            ccGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCa                                                           >  1:366668/1‑50 (MQ=255)                                                            ccGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCa                                                           <  2:366668/50‑1 (MQ=255)                                                            ccGGAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCggg                   <  2:713508/90‑1 (MQ=255)                                                              ggAATCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGa                 >  1:831446/1‑90 (MQ=255)                                                                 aTCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGtt              <  1:246581/90‑1 (MQ=255)                                                                 aTCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGtt              <  1:756425/90‑1 (MQ=255)                                                                 aTCCTGCAACGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGtt              <  1:996843/90‑1 (MQ=255)                                                                         aCGCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGTTATACCAGa      >  1:484043/1‑90 (MQ=255)                                                                           gCCAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGTTATACCAGAAt    >  2:1357605/1‑90 (MQ=255)                                                                             cAGTCCGGCAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGTTATACCAGAATAg  <  2:836321/90‑1 (MQ=255)                                                                                    |                                                                                   GAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGTTATACCAGAATAG  >  NZ_CP009273/1803118‑1803283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑