Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,939,453	G→C	A54G (GCC→GGC)	ruvB ←	Holliday junction branch migration DNA helicase RuvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,939,453	0	G	C	100.0%	116.4 / NA	36	A54G (GCC→GGC)	ruvB	Holliday junction branch migration DNA helicase RuvB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/21);  total (15/21)

AAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTT  >  NZ_CP009273/1939366‑1939538                                                                                        |                                                                                      aaaTTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAAC‑AATGATCGAGGCCAt                                                                                    <  2:190822/90‑1 (MQ=255)         gCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCcgcg                                                                             >  1:495996/1‑90 (MQ=255)          cccATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCgcgc                                                                            <  2:215030/90‑1 (MQ=255)          cccATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCgcgc                                                                            <  1:541146/90‑1 (MQ=255)          cccATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCAGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCgcgc                                                                            <  1:947631/90‑1 (MQ=255)             aTTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGt                                                                         >  1:850531/1‑90 (MQ=255)                  aTTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCgc                                                                    <  1:1066111/90‑1 (MQ=255)                      gCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCgctgct                                                                >  2:1439628/1‑90 (MQ=255)                       cGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCtt                                                               <  1:1478085/90‑1 (MQ=255)                        gACAATGTTGGCAAGCGTAGTTTTACGCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCttt                                                              >  2:1377687/1‑90 (MQ=255)                          cAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTtga                                                            >  1:1193897/1‑90 (MQ=255)                          cAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTtga                                                            <  1:1337005/90‑1 (MQ=255)                              gTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGaa                                                        <  2:71978/90‑1 (MQ=255)                                    aaGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAAtctc                                                  >  1:912464/1‑90 (MQ=255)                                      gCGTAGTTTTACCCAACGCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCa                                                >  1:1419655/1‑90 (MQ=255)                                      gCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCa                                                <  2:850531/90‑1 (MQ=255)                                      gCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCa                                                >  2:172309/1‑90 (MQ=255)                                        gTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATc                                              >  2:1022728/1‑90 (MQ=255)                                        gTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATc                                              <  2:234774/90‑1 (MQ=255)                                         tAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCt                                             <  1:553400/90‑1 (MQ=255)                                          aGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCtg                                            <  1:1240544/90‑1 (MQ=255)                                                aCCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTgaacg                                      >  1:1016553/1‑90 (MQ=255)                                                   cAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATcgc                                                                                 <  2:737756/44‑1 (MQ=255)                                                   cAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATcgc                                                                                 >  1:737756/1‑44 (MQ=255)                                                     aCCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCt                                 >  1:634571/1‑90 (MQ=255)                                                     aCCCCGGAGGACCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCt                                 >  2:1171961/1‑90 (MQ=255)                                                            aggaCCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTg                          <  1:662683/90‑1 (MQ=255)                                                            aggaCCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTg                          <  2:658688/90‑1 (MQ=255)                                                            aggaCCAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTg                          <  2:1075550/90‑1 (MQ=255)                                                                ccAAAAATCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCa                      >  2:1249876/1‑90 (MQ=255)                                                                     aaTCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATa                 <  1:1443345/90‑1 (MQ=255)                                                                      aTCAACAAATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATAc                <  2:206358/90‑1 (MQ=255)                                                                          acaaATGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCtt            <  2:1317584/90‑1 (MQ=255)                                                                              aTGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAg        <  1:1377687/90‑1 (MQ=255)                                                                              aTGATCGAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAg        <  2:1075595/90‑1 (MQ=255)                                                                                    gAGGCCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAAttt  >  1:1578605/1‑90 (MQ=255)                                                                                        |                                                                                      AAATTAACGCCCATTTCATTGGCGACAATGTTGGCAAGCGTAGTTTTACCCAACCCCGGAGGACCAAAAATCAACAAATGATCGAGGGCATCGCCGCGCAGTTTCGCTGCTTTGATGAAAATCTCCATCTGTGAACGAACCTGCGGCTGACCAACATACTCTTCCAGTAATTT  >  NZ_CP009273/1939366‑1939538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑