Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,576,634	A→G	R79R (CGA→CGG)	BW25113_RS17825 →	YrhA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,576,634	0	A	G	100.0%	102.1 / NA	32	R79R (CGA→CGG)	BW25113_RS17825	YrhA family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/10);  total (22/10)

ATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAA  >  NZ_CP009273/3576549‑3576719                                                                                      |                                                                                      aTGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGgttat                                                                                   >  2:537018/1‑90 (MQ=255) aTGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGgttat                                                                                   <  2:1566306/90‑1 (MQ=255)   gAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTAttt                                                                                 >  1:146810/1‑90 (MQ=255)      gTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGc                                                                              >  1:683298/1‑90 (MQ=255)       ttAGTATTCTTTTTAAGAATCATCCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGcc                                                                             <  1:609453/90‑1 (MQ=255)        tAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCct                                                                            >  2:690608/1‑90 (MQ=255)        tAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCct                                                                            >  1:1414362/1‑90 (MQ=255)        tAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCct                                                                            >  1:616864/1‑90 (MQ=255)        tAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCct                                                                            >  2:511649/1‑90 (MQ=255)        tAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCct                                                                            >  2:1044623/1‑90 (MQ=255)            aTTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCg                                                                        <  2:1051980/90‑1 (MQ=255)                tttttAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTc                                                                    >  1:1113014/1‑90 (MQ=255)                         aTCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCtt                                                           >  2:861142/1‑90 (MQ=255)                         aTCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCtt                                                           >  2:696937/1‑90 (MQ=255)                         aTCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCtt                                                           >  2:260248/1‑90 (MQ=255)                         aTCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCtt                                                           >  2:1006368/1‑90 (MQ=255)                                  aTTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTaaaa                                                  <  2:1080087/90‑1 (MQ=255)                                     aTCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAAcc                                               >  2:708036/1‑90 (MQ=255)                                       cTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCtt                                             >  2:1242378/1‑90 (MQ=255)                                        ttACTTTTTTAAGAGCAATGGATGGATTGGAAGTTAATGGATTACGGTTATTTAGTTTCTCGATTGCAGAATCTTCTGTTAAAAAACttt                                            >  1:944676/1‑90 (MQ=255)                                          aCTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCttttt                                          <  2:1388952/90‑1 (MQ=255)                                           ttttttgAAAAGCCGTGGATTGATTCGGGGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAAACTTCAGTTAAAAACCTTTTTg                                         >  1:791417/2‑90 (MQ=255)                                             tttttAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGcc                                       <  2:661469/90‑1 (MQ=255)                                               tttAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGt                                     >  1:1195708/1‑90 (MQ=255)                                                              tggatTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAg                      >  2:1167973/1‑90 (MQ=255)                                                               ggatTCGAAGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGa                     <  2:363181/90‑1 (MQ=255)                                                                      aaGTTAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAatg              <  1:710653/90‑1 (MQ=255)                                                                          tAATGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAAtgatga          >  2:517562/1‑90 (MQ=255)                                                                            aTGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGAtt        <  1:782283/90‑1 (MQ=255)                                                                            aTGGATTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGAtt        >  1:721661/1‑90 (MQ=255)                                                                                aTTACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCAt    >  2:48447/1‑90 (MQ=255)                                                                                 ttACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATa   <  2:1133852/90‑1 (MQ=255)                                                                                 ttACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATa   <  1:1167973/90‑1 (MQ=255)                                                                                  tACGGTTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATaa  >  2:1286313/1‑90 (MQ=255)                                                                                      |                                                                                      ATGAAGTTAGTATTCTTTTTAAGAATCAACCTGATTATCTTACTTTTTTAAGAGCAATGGATGGATTCGAAGTTAATGGATTACGATTATTTAGCCTCTCGATTCCAGAACCTTCAGTTAAAAACCTTTTTGCCGTAAATGAATTTTATAGAAATAATGATGATTTCATAA  >  NZ_CP009273/3576549‑3576719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑