| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,785,687 | G→C | G75G (GGC→GGG) | kbl ← | glycine C‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,785,687 | 0 | G | C | 96.5% | 184.0 / ‑1.1 | 58 | G75G (GGC→GGG) | kbl | glycine C‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base C (34/22); total (35/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
ATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAAA > NZ_CP009273/3785600‑3785772 | aTCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATccc < 1:237216/90‑1 (MQ=255) tCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGaaa < 2:271528/90‑1 (MQ=255) cATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAAcc < 2:312031/90‑1 (MQ=255) cAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTgaga > 1:104790/1‑90 (MQ=255) cAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTgaga > 2:351774/1‑90 (MQ=255) aGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTgag < 2:1514676/88‑1 (MQ=255) aGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTgag > 1:1514676/1‑88 (MQ=255) ggAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAAt > 1:610263/1‑90 (MQ=255) gAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATc < 1:887667/90‑1 (MQ=255) gccgccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCAtt > 1:503734/1‑90 (MQ=255) gccgccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCAtt > 2:1413590/1‑90 (MQ=255) gccgccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCAtt > 2:1559861/1‑90 (MQ=255) gccgccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCAtt > 1:1259543/1‑90 (MQ=255) ccgccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTc > 2:582333/1‑90 (MQ=255) ccgccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTc > 2:104111/1‑90 (MQ=255) gccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTccc > 1:300620/1‑90 (MQ=255) gtGTTAAAGCTCTTTATGGCGGCCCTGGGTCCCGCAAAAAAACCGCCCCGAACCCACCCCGAACCCGTGAGAACCCATTCCCGCCTTTgc < 1:940904/89‑1 (MQ=255) ttttCAAAGTCTTTATGGCTGTCCTGAGTGGCGCAAATAAAACGCCCCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTgcc < 2:1519358/88‑1 (MQ=255) cAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAACCGCCCCGAAGCCACCCCGAAACCGTGAGAATCCATTCCCGCCTTTGccgccg < 1:1413590/90‑1 (MQ=255) ctctTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAat < 2:22435/90‑1 (MQ=255) tctTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatc < 2:542739/90‑1 (MQ=255) tctTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatc < 2:522062/90‑1 (MQ=255) tctTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatc < 1:331784/90‑1 (MQ=255) ctTTATGGCTGTCCTGAGGGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcc > 2:940904/1‑89 (MQ=255) ttATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcaga < 1:578263/90‑1 (MQ=255) aTGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcagatc < 1:900241/90‑1 (MQ=255) aTGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcagatc < 1:72816/90‑1 (MQ=255) gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 2:693726/1‑90 (MQ=255) gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGGGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 1:771640/1‑90 (MQ=255) gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 2:1443894/1‑90 (MQ=255) gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 2:1344437/1‑90 (MQ=255) gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 2:8975/1‑90 (MQ=255) gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 2:183336/1‑90 (MQ=255) gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 1:1225138/1‑90 (MQ=255) gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 2:125159/1‑90 (MQ=255) gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 1:1457007/1‑90 (MQ=255) gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 1:1470409/1‑90 (MQ=255) gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 1:201369/1‑90 (MQ=255) gTCCTGAGCGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg > 2:100873/1‑90 (MQ=255) ccTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatgat > 1:975922/1‑90 (MQ=255) gTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGcc < 2:300620/90‑1 (MQ=255) gTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGcc > 1:466212/1‑90 (MQ=255) gTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGcc > 2:641283/1‑90 (MQ=255) ccGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGc > 2:1147856/1‑90 (MQ=255) ccGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGc > 1:883425/1‑90 (MQ=255) ccGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGc > 1:395842/1‑90 (MQ=255) gCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc > 1:784740/1‑90 (MQ=255) gCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc < 1:486104/90‑1 (MQ=255) aaTAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCgag > 2:182418/1‑90 (MQ=255) tAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc > 1:404673/1‑85 (MQ=255) tAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc < 2:404673/85‑1 (MQ=255) tAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGAt < 2:975922/90‑1 (MQ=255) aaccGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAg < 2:1099546/87‑1 (MQ=255) aaCGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAgt > 2:386060/1‑90 (MQ=255) cACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAgttgtt < 2:784740/90‑1 (MQ=255) cACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAgttgtt > 1:1369669/1‑90 (MQ=255) cACCGAAGCCATCCCGAAACCGTGAAATTCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAgttgtt < 1:1487604/90‑1 (MQ=255) gCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACaa < 2:435266/90‑1 (MQ=255) ccATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACaaa < 2:1259543/90‑1 (MQ=255) | ATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAAA > NZ_CP009273/3785600‑3785772 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |