Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,896,621	A→G	D147D (GAT→GAC)	bglB ←	6‑phospho‑beta‑glucosidase BglB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,896,621	0	A	G	100.0%	192.8 / NA	62	D147D (GAT→GAC)	bglB	6‑phospho‑beta‑glucosidase BglB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/19);  total (43/19)

ACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCTT  >  NZ_CP009273/3896536‑3896707                                                                                      |                                                                                       aCATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGa                                                                                    >  1:785777/1‑90 (MQ=255)   aTGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATg                                                                                  <  2:785777/90‑1 (MQ=255)   aTGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATg                                                                                  <  1:1184766/90‑1 (MQ=255)   aTGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATg                                                                                  >  2:1306338/1‑90 (MQ=255)   aTGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATg                                                                                  <  2:1097733/90‑1 (MQ=255)   aTGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATg                                                                                  >  1:1546886/1‑90 (MQ=255)   aTGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATg                                                                                  <  1:415699/90‑1 (MQ=255)    tGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGa                                                                                 <  1:1232774/90‑1 (MQ=255)    tGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGa                                                                                 >  2:598082/1‑90 (MQ=255)        gATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGc                                                                             <  2:485866/90‑1 (MQ=255)         aTTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCt                                                                            >  1:730107/1‑90 (MQ=255)             cATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGAt                                                                        <  1:560088/90‑1 (MQ=255)              aTTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGAtt                                                                       <  2:714362/90‑1 (MQ=255)               ttAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTa                                                                      <  1:491433/90‑1 (MQ=255)                    cgccAGCCATACCGCCTTTTTTTGTTGGTAGCGAGAAAAGCCCGTGGGGGCGTAATGCTCGAGGTGGTCGATGCCCGCTCGATTACCCCa                                                                 <  1:187629/87‑1 (MQ=255)                       cAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAAcc                                                              >  1:551816/1‑90 (MQ=255)                       cAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAAcc                                                              >  2:24694/1‑90 (MQ=255)                       cAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAAcc                                                              >  2:1211824/1‑90 (MQ=255)                       cAGCAATACCGCCTTTTTATGTGGGTAGCGAGAAAAGCCCGGGGGGGCGTAATGCTCGAAGTGGTCGATGCCCGCTCGATTAGCCCAAcc                                                              <  1:537304/90‑1 (MQ=255)                              aaCGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAg                                                       >  2:1467380/1‑90 (MQ=255)                                  ccACTTTATGTTGGTAGCGAGTAGAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGtttt                                                   >  1:278028/1‑90 (MQ=255)                                   cACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGttttt                                                  >  1:1303401/1‑90 (MQ=255)                                   cACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGttttt                                                  >  2:1468127/1‑90 (MQ=255)                                    aCTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTc                                                 >  1:999618/1‑90 (MQ=255)                                        tATGTTGGTGGCGAGAAAAGACCGCGCGGGTATAATGCTCGACGTGGTCGATGACCTCCCTATTCGCCCAACCGCCGTAGTTTTTCACCa                                             <  2:330536/90‑1 (MQ=255)                                        tATGTGGGTAGGGAGTAAAGACCGGGGGGGCGTAATGCTGGAAGTGGTCGTGGCCCGCTCGATTAGCCCACCCGCCGTAGTTTTTCACCa                                             <  2:756034/90‑1 (MQ=255)                                           gTTGGTGGCGAAAAAAGCCGGGGGGGGCGTATTGCTCGAAGTGGTCGATGCCCCCTCGTTTACCCCAACCGCCGTAGTTTTTCACCAGcc                                          <  1:585954/90‑1 (MQ=255)                                           gTTGGTAGCGAGTAAAGCCCGGGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGcc                                          <  2:248119/90‑1 (MQ=255)                                             tGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCa                                        >  1:682616/1‑90 (MQ=255)                                              ggTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCat                                       >  2:1406823/1‑90 (MQ=255)                                               gTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCata                                      >  1:715577/1‑90 (MQ=255)                                                 aGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATg                                    >  2:1169160/1‑90 (MQ=255)                                                   cGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGc                                  >  2:1452163/1‑90 (MQ=255)                                                   cGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGc                                  >  2:330405/1‑90 (MQ=255)                                                    gagaAAAGCCGGGGCGGGCGAAATGCGCGAGGGGGTCGATCCCCCCTAGATCCCCCAAACCGCCGTAGTTTTTCACCAGCCCATATGGCa                                 <  1:1211824/90‑1 (MQ=255)                                                    gAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCa                                 >  1:1489235/1‑90 (MQ=255)                                                        aaaGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTc                             >  2:382967/1‑90 (MQ=255)                                                        aaaGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTc                             >  2:871922/1‑90 (MQ=255)                                                           gACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTa                          >  1:424182/1‑90 (MQ=255)                                                              cGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATg                       >  1:516320/1‑90 (MQ=255)                                                              cGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATg                       >  1:1038900/1‑90 (MQ=255)                                                              cGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATg                       >  2:416060/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:1472034/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:1014258/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:861668/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  1:266761/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:754719/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  1:326176/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:204690/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:344998/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:287843/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  1:699979/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  1:929153/1‑90 (MQ=255)                                                               gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg                      >  2:214523/1‑90 (MQ=255)                                                                tGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATggg                     >  1:520847/1‑90 (MQ=255)                                                                tGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATggg                     >  1:377314/1‑90 (MQ=255)                                                                   gggCGTAATGCTCGAGGGGGTCGATGCCCGCTCTATTACCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATa                  <  1:529907/90‑1 (MQ=255)                                                                   gggCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATa                  <  2:1489235/90‑1 (MQ=255)                                                                              tCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGc       >  2:715299/1‑90 (MQ=255)                                                                              tCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGc       >  1:1054252/1‑90 (MQ=255)                                                                                  aGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCt   >  2:815920/1‑90 (MQ=255)                                                                                   gTGGTCGATCCCCGCTCGTTTCCCCCACCCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCtt  <  2:1140857/90‑1 (MQ=255)                                                                                      |                                                                                       ACATGTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATAACGTTACCAGCGGCTT  >  NZ_CP009273/3896536‑3896707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑