Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,901,121	A→G	D202D (GAT→GAC)	pstB ←	phosphate ABC transporter ATP‑binding protein PstB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,901,121	0	A	G	100.0%	275.0 / NA	81	D202D (GAT→GAC)	pstB	phosphate ABC transporter ATP‑binding protein PstB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/42);  total (39/42)

ATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGC  >  NZ_CP009273/3901035‑3901205                                                                                       |                                                                                     atcaatTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTcc                                                                                   <  1:1469418/90‑1 (MQ=255) atcaatTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTcc                                                                                   <  1:1335324/90‑1 (MQ=255) atcaatTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGGGACGATCACCACGGTGTAGTcc                                                                                   >  1:1039480/1‑90 (MQ=255)  tcaatTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCt                                                                                  <  1:1145697/90‑1 (MQ=255)       tCGCCCAGGTACATAAACGCCGTGTGGTCGGAACACCGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc                                                                             <  1:696703/90‑1 (MQ=255)       tCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc                                                                             <  2:836209/90‑1 (MQ=255)       tCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc                                                                             <  2:671272/90‑1 (MQ=255)       tCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc                                                                             >  2:529844/1‑90 (MQ=255)       tCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc                                                                             <  2:1029705/90‑1 (MQ=255)         gCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAg                                                                           >  2:1127786/1‑90 (MQ=255)         gCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAg                                                                           <  1:789719/90‑1 (MQ=255)         gCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAg                                                                           <  2:1228014/90‑1 (MQ=255)                   aTAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATc                                                                 <  1:717998/90‑1 (MQ=255)                   aTAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATc                                                                 >  2:377395/1‑90 (MQ=255)                   aTAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATc                                                                 <  1:1109406/90‑1 (MQ=255)                   aTAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATc                                                                 <  1:393712/90‑1 (MQ=255)                      aaCGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGc                                                              <  1:1042896/90‑1 (MQ=255)                      aaCGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGc                                                              <  1:75756/90‑1 (MQ=255)                      aaCGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGc                                                              <  2:640314/90‑1 (MQ=255)                      aaCGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGc                                                              <  1:377395/90‑1 (MQ=255)                       aCGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGct                                                             >  2:134606/1‑90 (MQ=255)                       aCGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGct                                                             >  1:1491235/1‑90 (MQ=255)                        cGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctc                                                            >  1:946321/1‑90 (MQ=255)                        cGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctc                                                            >  2:189803/1‑90 (MQ=255)                        cGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGc                                                              >  2:979444/1‑88 (MQ=255)                        cGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGc                                                              <  1:979444/88‑1 (MQ=255)                         gCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctct                                                           <  2:1538670/90‑1 (MQ=255)                         gCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctct                                                           >  1:1323476/1‑90 (MQ=255)                           cGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTc                                                         >  2:522689/1‑90 (MQ=255)                            gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATTACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCa                                                        >  2:1263877/1‑90 (MQ=255)                            gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCa                                                        >  2:1520152/1‑90 (MQ=255)                            gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCa                                                        >  2:655610/1‑90 (MQ=255)                            gtgtGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCa                                                        >  1:1189265/1‑90 (MQ=255)                               tgGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATa                                                     <  2:340625/90‑1 (MQ=255)                               tgGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATa                                                     <  1:633299/90‑1 (MQ=255)                               tgGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATa                                                     <  2:1289152/90‑1 (MQ=255)                                  tCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGc                                                  <  1:731565/90‑1 (MQ=255)                                    ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                                <  2:50415/90‑1 (MQ=255)                                    ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                                <  2:1527900/90‑1 (MQ=255)                                    ggAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGccc                                                <  1:263434/90‑1 (MQ=255)                                     gAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCg                                               <  1:1072664/90‑1 (MQ=255)                                      aacaacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGc                                                                                <  2:1112369/56‑1 (MQ=255)                                      aacaacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGc                                                                                >  1:1112369/1‑56 (MQ=255)                                       acaacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGt                                             <  1:1208564/90‑1 (MQ=255)                                        caacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                                            <  2:79106/90‑1 (MQ=255)                                        caacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                                            <  2:396707/90‑1 (MQ=255)                                        caacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                                            <  2:907493/90‑1 (MQ=255)                                        caacGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTa                                            <  1:1085849/90‑1 (MQ=255)                                          acGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTaga                                          >  2:460408/1‑90 (MQ=255)                                             cgcAGCCTGCTGCATGTTGTGGGTGACGATTACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGAt                                       <  1:1263877/90‑1 (MQ=255)                                             cgcAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGAt                                       <  1:655610/90‑1 (MQ=255)                                               cAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAg                                     >  2:593478/1‑90 (MQ=255)                                                  ccTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctct                                                           <  1:1144590/65‑1 (MQ=255)                                                  ccTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGctct                                                           >  2:1144590/1‑65 (MQ=255)                                                    tgctgcATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCg                                <  1:1520152/90‑1 (MQ=255)                                                      ctgcATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc                                                                             >  2:79615/1‑43 (MQ=255)                                                      ctgcATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTc                                                                             <  1:79615/43‑1 (MQ=255)                                                        gcATGTTGTGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAgcg                            <  1:189803/90‑1 (MQ=255)                                                           tgttgtGGGTGACGATCACCACGTTGGAGTCCTGCTTCAGTTCGGGGATCAGCTCTTCAATACGCCCGGCAGAGATAAGGTCGAGCGCCg                         >  1:95044/1‑90 (MQ=255)                                                           tgttgtGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCg                         >  1:1580691/1‑90 (MQ=255)                                                           tgttgtGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCg                         >  1:536950/1‑90 (MQ=255)                                                           tgttgtGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCg                         >  2:149957/1‑90 (MQ=255)                                                           tgttgtGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCg                         >  2:1072609/1‑90 (MQ=255)                                                             ttgtGGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGaa                       >  2:603135/1‑90 (MQ=255)                                                               gtgGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAaca                     >  2:81321/1‑90 (MQ=255)                                                               gtgGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAaca                     >  1:460794/1‑90 (MQ=255)                                                                tgGGTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAacac                    >  2:806429/1‑90 (MQ=255)                                                                  ggTGACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACgg                  >  2:783215/1‑90 (MQ=255)                                                                  ggTGACGATCACCACAGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACgg                  >  2:147780/1‑90 (MQ=255)                                                                     gACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTtc               >  2:1210316/1‑90 (MQ=255)                                                                     gACGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTtc               >  2:108934/1‑90 (MQ=255)                                                                       cGATCACCACGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTtcgt             <  2:47055/90‑1 (MQ=255)                                                                                cGGTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagcagca    >  1:8543/1‑90 (MQ=255)                                                                                 ggTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagcagcag   >  2:992881/1‑90 (MQ=255)                                                                                 ggTGTAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagcagcag   <  1:1135620/90‑1 (MQ=255)                                                                                  gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAgcgc                           >  1:1100244/1‑65 (MQ=255)                                                                                  gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAgcgc                           <  2:1100244/65‑1 (MQ=255)                                                                                  gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTtcgt             >  1:390868/1‑79 (MQ=255)                                                                                  gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTtcgt             <  2:390868/79‑1 (MQ=255)                                                                                  gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagcagcagc  >  1:1265810/1‑90 (MQ=255)                                                                                  gtgtAGTCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGagcagcagc  >  1:79467/1‑90 (MQ=255)                                                                                       |                                                                                     ATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGAACACGGTTCGTCGAGCAGCAGC  >  NZ_CP009273/3901035‑3901205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑