Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,527,151	T→C	Q87Q (CAA→CAG)	nanS ←	9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,527,151	0	T	C	100.0%	310.1 / NA	92	Q87Q (CAA→CAG)	nanS	9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/49);  total (43/49)

ATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTTTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTAA  >  NZ_CP009273/4527068‑4527236                                                                                    |                                                                                      aTAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCtgatga                                                                                 >  2:675485/1‑90 (MQ=255) aTAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCtgatga                                                                                 >  2:119284/1‑90 (MQ=255)    aGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTc                                                                              <  1:64617/90‑1 (MQ=255)     gAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCg                                                                             <  2:1478239/90‑1 (MQ=255)        tCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTg                                                                          >  2:736978/1‑90 (MQ=255)         cccTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGc                                                                         >  1:995617/1‑90 (MQ=255)          ccTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGcc                                                                        <  1:1233222/90‑1 (MQ=255)           cTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGc                                                                         >  1:371471/1‑88 (MQ=255)           cTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGc                                                                         <  2:371471/88‑1 (MQ=255)           cTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCa                                                                       >  1:1176794/1‑90 (MQ=255)           cTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCa                                                                       >  2:1442271/1‑90 (MQ=255)            tGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCag                                                                      <  1:1172004/90‑1 (MQ=255)            tGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCag                                                                      <  1:206525/90‑1 (MQ=255)                ttATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa                                                                  <  2:1402391/90‑1 (MQ=255)                ttATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa                                                                  <  2:1083363/90‑1 (MQ=255)                 tATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGAt                                                                 <  1:1481429/90‑1 (MQ=255)                  aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                <  2:1313760/90‑1 (MQ=255)                  aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                <  2:911689/90‑1 (MQ=255)                  aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                <  1:552658/90‑1 (MQ=255)                  aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                <  2:735162/90‑1 (MQ=255)                  aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                >  1:500920/1‑90 (MQ=255)                  aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                <  2:873282/90‑1 (MQ=255)                  aTCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                >  1:173320/1‑90 (MQ=255)                     aGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTg                                                             <  1:771074/90‑1 (MQ=255)                       gAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGAt                                                           >  1:996249/1‑90 (MQ=255)                         aTGAAGGGCAGTAATTGCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGGACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATaa                                                         >  1:815870/1‑90 (MQ=255)                           gAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAAcc                                                       <  2:55042/90‑1 (MQ=255)                           gAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAAcc                                                       <  2:1540835/90‑1 (MQ=255)                           gAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAAcc                                                       <  2:841459/90‑1 (MQ=255)                           gAAGGGCAGTAATTTCCGTGCAATATGCAGGGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAAcc                                                       <  2:1514830/90‑1 (MQ=255)                                gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  2:1322623/90‑1 (MQ=255)                                gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  1:675485/90‑1 (MQ=255)                                gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  2:441353/90‑1 (MQ=255)                                gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  2:1050067/90‑1 (MQ=255)                                gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  1:752594/90‑1 (MQ=255)                                gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  1:203855/90‑1 (MQ=255)                                gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  1:383218/90‑1 (MQ=255)                                gCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  2:1009958/90‑1 (MQ=255)                                 cAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCat                                                 >  1:1387791/1‑90 (MQ=255)                                  aGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCata                                                <  1:277980/90‑1 (MQ=255)                                  aGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCata                                                <  2:435186/90‑1 (MQ=255)                                  aGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCata                                                <  1:984174/90‑1 (MQ=255)                                  aGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCata                                                <  2:1062979/90‑1 (MQ=255)                                        ttCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                <  1:274319/68‑1 (MQ=255)                                        ttCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                >  2:274319/1‑68 (MQ=255)                                           cGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGGTAACCCTGCATATCCTGAACa                                       <  2:163391/90‑1 (MQ=255)                                           cGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACa                                       <  1:348585/90‑1 (MQ=255)                                             tGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATc                                     <  2:1209846/90‑1 (MQ=255)                                                 atatGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  >  2:591045/1‑73 (MQ=255)                                                 atatGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  1:591045/73‑1 (MQ=255)                                                 atatGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCgtgt                                 >  1:199217/1‑90 (MQ=255)                                                   atGCAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTgg                               >  1:1505287/1‑90 (MQ=255)                                                      cAGTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCa                            >  2:503477/1‑90 (MQ=255)                                                        gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa                                                                  >  2:36540/1‑50 (MQ=255)                                                        gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGa                                                                  <  1:36540/50‑1 (MQ=255)                                                        gTGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGGGATAACCCTGCATATCCTGAACATCGTGTGGGCAGt                          >  1:1177048/1‑90 (MQ=255)                                                         tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCata                                                <  1:896404/67‑1 (MQ=255)                                                         tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCata                                                >  2:896404/1‑67 (MQ=255)                                                         tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTg                         >  2:636434/1‑90 (MQ=255)                                                         tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTg                         >  1:1027073/1‑90 (MQ=255)                                                         tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTg                         >  1:1471255/1‑90 (MQ=255)                                                         tGCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCG‑TGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGa                        >  1:1519688/1‑90 (MQ=255)                                                          gCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                >  2:89371/1‑50 (MQ=255)                                                          gCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                <  1:89371/50‑1 (MQ=255)                                                          gCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  >  2:708908/1‑64 (MQ=255)                                                          gCCTGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  1:708908/64‑1 (MQ=255)                                                             tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                >  1:440989/1‑47 (MQ=255)                                                             tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATg                                                                <  2:440989/47‑1 (MQ=255)                                                             tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  <  1:566954/61‑1 (MQ=255)                                                             tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCa                                                  >  2:566954/1‑61 (MQ=255)                                                             tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc                     <  1:847132/90‑1 (MQ=255)                                                             tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc                     >  2:847132/1‑90 (MQ=255)                                                             tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc                     >  1:1358722/1‑90 (MQ=255)                                                             tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc                     >  2:1410404/1‑90 (MQ=255)                                                             tGGCCAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTc                     >  2:162750/1‑90 (MQ=255)                                                                ccAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGt                  <  1:1294587/90‑1 (MQ=255)                                                                ccAACGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGt                  <  1:12404/90‑1 (MQ=255)                                                                  aaCGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCgtgt                                 >  1:725895/1‑73 (MQ=255)                                                                  aaCGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCgtgt                                 <  2:725895/73‑1 (MQ=255)                                                                  aaCGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGGGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTgg                >  1:1217410/1‑90 (MQ=255)                                                                   aCGGTGCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGa               >  1:1056160/1‑90 (MQ=255)                                                                        gCCGTACTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGaataat          >  2:158229/1‑90 (MQ=255)                                                                             aCTGTGTCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGt     <  2:1010018/90‑1 (MQ=255)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAAcc                                                       <  1:1335355/37‑1 (MQ=38)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAAcc                                                       >  2:1335355/1‑37 (MQ=38)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  1:647331/1‑90 (MQ=255)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  2:647910/1‑90 (MQ=255)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  2:1518039/1‑90 (MQ=255)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  2:273207/1‑90 (MQ=255)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  1:954865/1‑90 (MQ=255)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  <  1:273207/90‑1 (MQ=255)                                                                                gtgtCTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTaa  >  1:994080/1‑90 (MQ=255)                                                                                    |                                                                                      ATAAGAATCCCTGCATTATCAGGAATGAAGGGCAGTAATTTCCGTGCAATATGCAGTGCCTGGCCAACGGTGCCGTACTGTGTTTGATGATTCGTTGCCAGAGGATGGTGATAACCCTGCATATCCTGAACATCGTGTGGGCAGTGAGTCAGTGGAATAATGTCGTTAA  >  NZ_CP009273/4527068‑4527236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑