Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	539,975	A→G	D258D (GAT→GAC)	allC ←	allantoate deiminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	539,975	0	A	G	100.0%	166.8 / NA	51	D258D (GAT→GAC)	allC	allantoate deiminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/14);  total (37/14)

AATCAATGGTGAACGTGGTTTTACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGATCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCACGACGATAACCCATCGGC  >  NZ_CP009273/539890‑540059                                                                                      |                                                                                     aatcaatGGTGAACGTGGTTTTACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGc                                                                                  >  2:288272/1‑90 (MQ=255) aatcaatGGTGAACGTGGTTTTACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGc                                                                                  >  2:665736/1‑90 (MQ=255)      atGGTGAACGTGGTTTTACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGTCAAAGGTCAGAACCAGCGGGTCGCCCATc                                                                             >  2:297797/1‑90 (MQ=255)           gAACGTGGTTTTACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCtttt                                                                        >  2:410795/1‑90 (MQ=255)           gAACGTGGTTTTACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCtttt                                                                        >  1:238810/1‑90 (MQ=255)           gAACGTGGTTTTACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCACCCtttc                                                                        >  1:1387293/1‑89 (MQ=255)            aaCGTGGTTTTACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTc                                                                       >  2:411105/1‑90 (MQ=255)                tGGTTTTACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCtt                                                                   >  1:56626/1‑90 (MQ=255)                   tcttACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCttttt                                                                <  1:1341205/88‑1 (MQ=255)                     ttACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTcg                                                              >  1:1119717/1‑90 (MQ=255)                       aCCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTcgac                                                            >  1:179752/1‑90 (MQ=255)                       aCCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTcgac                                                            >  2:555816/1‑90 (MQ=255)                        cccGGCCCCCCATTTTCCCTTTTCGGGCGGGGGTCCTCCTTGCCAAAGGTCAGAACCAGGGGGTCGGCCATCCTTTTCGCTTTTTcgacc                                                           <  2:1387293/90‑1 (MQ=255)                          cGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTcgaccga                                                         >  1:1466272/1‑90 (MQ=255)                            gCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACt                                                       >  2:186418/1‑90 (MQ=255)                            gCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACt                                                       >  2:1261748/1‑90 (MQ=255)                                 acaTTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATgg                                                  >  1:568250/1‑90 (MQ=255)                                      tACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCg                                                                      >  2:49255/1‑65 (MQ=255)                                      tACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCg                                                                      <  1:49255/65‑1 (MQ=255)                                      tACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAAt                                             >  2:900478/1‑90 (MQ=255)                                         cGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCg                                          <  1:761770/90‑1 (MQ=255)                                         cGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCg                                          >  2:76638/1‑90 (MQ=255)                                         cGTATTCGGGCGCAGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCg                                          >  1:540222/1‑90 (MQ=255)                                            aTTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACt                                       >  2:582046/1‑90 (MQ=255)                                               cGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGaa                                    <  1:158547/90‑1 (MQ=255)                                                gggCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGaaa                                   <  1:751389/90‑1 (MQ=255)                                                      ggCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTaa                             <  2:1119717/90‑1 (MQ=255)                                                      ggCTCTACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTaa                             >  1:709279/1‑90 (MQ=255)                                                        ctctACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAAc                           <  2:568250/90‑1 (MQ=255)                                                        ctctACTTTGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAAc                           <  1:1206924/90‑1 (MQ=255)                                                               ttGCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCa                    >  1:398485/1‑90 (MQ=255)                                                                 gCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCacg                  >  2:1530439/1‑90 (MQ=255)                                                                 gCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCacg                  >  2:1419838/1‑90 (MQ=255)                                                                 gCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCacg                  >  2:1358670/1‑90 (MQ=255)                                                                 gCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCacg                  >  2:1320332/1‑90 (MQ=255)                                                                 gCCAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCacg                  <  1:900478/90‑1 (MQ=255)                                                                   cAAAGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCacgac                >  2:511001/1‑90 (MQ=255)                                                                    aaaGGTCAGAACCAGCGGGACGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCacgacg               >  2:798674/1‑90 (MQ=255)                                                                      aGGTCAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCACGACGAt             >  2:1155027/1‑90 (MQ=255)                                                                          cAGAACCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCACGACGATAAcc         >  1:1374158/1‑90 (MQ=255)                                                                              aCCAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCACGACGATAACCCATc     <  1:763141/90‑1 (MQ=255)                                                                               ccAGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCACGACGATAACCCATCg    <  2:56626/90‑1 (MQ=255)                                                                                 aGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGa                                     >  1:175029/1‑55 (MQ=255)                                                                                 aGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGa                                     <  2:175029/55‑1 (MQ=255)                                                                                 aGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAAc                           <  2:613908/65‑1 (MQ=255)                                                                                 aGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAAc                           >  1:613908/1‑65 (MQ=255)                                                                                 aGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCACGACGATAACCCATCGGc  >  2:506326/1‑90 (MQ=255)                                                                                 aGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCACGACGATAACCCATCGGc  >  2:416867/1‑90 (MQ=255)                                                                                 aGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCACGACGATAACCCATCGGc  >  2:662696/1‑90 (MQ=255)                                                                                 aGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCACGACGATAACCCATCGGc  >  1:699138/1‑90 (MQ=255)                                                                                 aGCGGGTCGCCCATCCTTTTCGCTTTTTCGACCGACGGATGGCAAATGCGACTGAAAGCGTAAACTGTATCACGACGATAACCCATCGGc  >  1:438952/1‑90 (MQ=255)                                                                                      |                                                                                     AATCAATGGTGAACGTGGTTTTACCCGGCACCACATTTACCGTATTCGGGCGCGGCTCTACTTTGCCAAAGGTCAGAACCAGCGGATCGCCCATCCTTTTCGCTTTTTCGACCGACTGATGGCAAATGCGACTGAAAGCGTAAACTGTATCACGACGATAACCCATCGGC  >  NZ_CP009273/539890‑540059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑