Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	50,280	T→C	F153S (TTT→TCT)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	50,280	0	T	C	100.0%	165.6 / NA	50	F153S (TTT→TCT)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/30);  total (20/30)

TTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTA  >  NZ_CP009273/50194‑50366                                                                                       |                                                                                       tttCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTga                                                                                     <  1:1201357/90‑1 (MQ=255)       ggATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCt                                                                               >  2:1046375/1‑90 (MQ=255)       ggATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCt                                                                               >  1:1414905/1‑90 (MQ=255)       ggATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCt                                                                               >  2:35959/1‑90 (MQ=255)       ggATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCt                                                                               >  1:863452/1‑90 (MQ=255)             cGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTATGGAgcg                                                                         <  1:1494184/90‑1 (MQ=255)                 ccGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGcggcgg                                                                     >  2:62599/1‑90 (MQ=255)                    gATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGtga                                                                  >  1:1216566/1‑88 (MQ=255)                       gACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAAttt                                                               <  2:918079/90‑1 (MQ=255)                             gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGtata                                                         <  2:1492521/90‑1 (MQ=255)                             gAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGtata                                                         >  2:447597/1‑90 (MQ=255)                                    tATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTa                                                  <  1:732163/90‑1 (MQ=255)                                         aGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCt                                             <  1:81160/90‑1 (MQ=255)                                             aaTTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTTCGGCTAacg                                         >  1:170914/1‑90 (MQ=255)                                             aaTTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTTTATAAAATTTTCGGCTAacg                                         >  1:400401/1‑90 (MQ=255)                                                         cTGATGCGCAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACg                             >  1:353121/1‑90 (MQ=255)                                                               cgcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTc                       <  2:1359576/90‑1 (MQ=255)                                                               cgcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTc                       <  1:569656/90‑1 (MQ=255)                                                               cgcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTc                       <  2:439093/90‑1 (MQ=255)                                                                gcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTTTATAAAATTTTCGGGTAACGCCGGGGGCGGCGCCCGTcg                      >  1:1278094/1‑90 (MQ=255)                                                                gcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTcg                      >  2:625646/1‑90 (MQ=255)                                                                gcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTcg                      <  2:820028/90‑1 (MQ=255)                                                                gcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTTTATAGAATTTACGGCTAGCGCCGGGTGCGGCgccggccg                      >  1:1517483/1‑90 (MQ=255)                                                                gcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTGTATAGAATTTACGGCTAGCGCCGGGAGCGACGCCGGGcg                      >  2:356696/1‑90 (MQ=255)                                                                gcAGAACTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGGAATTTTGTATAGAAATTACGGCTACCGCCCGATTCGACCCCCGTcg                      >  2:1028116/1‑90 (MQ=255)                                                                    aaCTCTCACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTc                  <  1:756217/90‑1 (MQ=255)                                                                        ctcACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCCGATGCGACGCCCGGCGCGGCtttt              >  1:435048/1‑88 (MQ=255)                                                                         tcACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTACGGCTAACGCCGGGTGGGACGCCGGGCGCGGCTTATc             >  2:1514925/1‑90 (MQ=255)                                                                         tcACAGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATc             >  2:342927/1‑90 (MQ=255)                                                                            caGCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCgg          <  1:212644/90‑1 (MQ=255)                                                                              gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc        <  2:433113/90‑1 (MQ=255)                                                                              gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc        <  1:1099475/90‑1 (MQ=255)                                                                              gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc        <  2:728768/90‑1 (MQ=255)                                                                              gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc        <  2:88697/90‑1 (MQ=255)                                                                              gCTATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc        <  1:62599/90‑1 (MQ=255)                                                                              gCTATTGCTCTAAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc        <  1:1341518/90‑1 (MQ=255)                                                                                tATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc        <  1:515049/88‑1 (MQ=255)                                                                                tATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGcc        >  2:515049/1‑88 (MQ=255)                                                                                tATTGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCtt      <  2:737528/90‑1 (MQ=255)                                                                                acaggCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCtt      <  1:1209161/86‑1 (MQ=255)                                                                                  ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc    <  2:123938/90‑1 (MQ=255)                                                                                  ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc    <  2:1216566/90‑1 (MQ=255)                                                                                  ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc    <  2:448429/90‑1 (MQ=255)                                                                                  ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc    <  1:35959/90‑1 (MQ=255)                                                                                  ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc    <  1:1472477/90‑1 (MQ=255)                                                                                  ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc    <  2:875305/90‑1 (MQ=255)                                                                                  ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc    <  1:1101136/90‑1 (MQ=255)                                                                                  ttGCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTcc    >  2:9957/1‑90 (MQ=255)                                                                                    gCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCta  <  1:60709/90‑1 (MQ=255)                                                                                    gCTCTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCta  <  1:1065824/90‑1 (MQ=255)                                                                                       |                                                                                       TTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTCTTATCCGGCCTTCCTA  >  NZ_CP009273/50194‑50366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑