| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 601,986 | A→G | F285S (TTC→TCC) | ybdK ← | YbdK family carboxylate‑amine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 601,986 | 0 | A | G | 97.7% | 136.7 / NA | 43 | F285S (TTC→TCC) | ybdK | YbdK family carboxylate‑amine ligase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (19/23); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAAT > NZ_CP009273/601900‑602071 | aTCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAAc < 2:1423737/90‑1 (MQ=255) cAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGtt < 2:1509368/90‑1 (MQ=255) cAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGtt < 1:1266196/90‑1 (MQ=255) cAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGtt < 1:164072/90‑1 (MQ=255) tCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATAc > 1:410215/1‑90 (MQ=255) ttAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATAcagca < 1:422238/90‑1 (MQ=255) cGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTa < 2:1044631/90‑1 (MQ=255) cGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTa < 2:104388/90‑1 (MQ=255) tCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATc > 1:951211/1‑90 (MQ=255) gacgaTCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTa > 1:362970/1‑85 (MQ=255) gacgaTCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTa < 2:362970/85‑1 (MQ=255) gacgaTCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCtt < 1:271247/90‑1 (MQ=255) gacgaTCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCtt < 2:20193/90‑1 (MQ=255) tcCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTg < 2:652622/90‑1 (MQ=255) gtgtGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGa < 1:900305/87‑1 (MQ=255) gtgtGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGa > 2:900305/1‑87 (MQ=255) gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt > 2:1159105/1‑90 (MQ=255) gtgCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGttt > 2:935930/1‑90 (MQ=255) gCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTaa > 1:1530930/1‑90 (MQ=255) cGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTaaa > 1:483447/1‑90 (MQ=255) gATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACg > 2:1241866/1‑90 (MQ=255) cGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCt < 2:342077/75‑1 (MQ=255) cGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCt > 1:342077/1‑75 (MQ=255) atgaCGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTcc < 1:1189722/90‑1 (MQ=255) gaCGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGt > 2:781519/1‑90 (MQ=255) gaCGCCTTCAAGCCCAAAGCCGCAGGGCCTGGAACGGGTTAAATTTTTAAACAAGTTATTTTTTTTCCTGATGTTTAACGGGGGTTTCCg < 1:39705/90‑1 (MQ=255) aCGCCTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTc < 2:1417194/90‑1 (MQ=255) ccTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGt > 1:1168733/1‑90 (MQ=255) cTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTa > 1:12626/1‑52 (MQ=255) cTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTa < 2:12626/52‑1 (MQ=255) cTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTa > 2:1340592/1‑90 (MQ=255) cTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTa > 2:898111/1‑90 (MQ=255) cTTCAAGCCCATAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTa > 2:39705/1‑90 (MQ=255) aTAGCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGc > 1:491806/1‑90 (MQ=255) gCGACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGt < 2:785896/90‑1 (MQ=255) gACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAg > 2:1131576/1‑90 (MQ=255) gACA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAg > 2:948351/1‑90 (MQ=255) cA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGcc < 1:265299/90‑1 (MQ=255) cA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGcc < 2:761619/90‑1 (MQ=255) cA‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGcc < 2:1374667/90‑1 (MQ=255) a‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCt < 2:703511/90‑1 (MQ=255) a‑GGCCTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCt < 2:1271362/90‑1 (MQ=255) cTGGGAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAAt < 1:935930/90‑1 (MQ=255) | ATCGCAAGGTATCTTCCGTTAGCGGTCGACGATCTCCAGTGTGCGGATCGGTGATGACGCCTTCAAGCCCATAGCGACA‑GGCCTGGAAACGGTTGAATTTATACAGCAGGTAATCTTTTTCCTGATGTTTAAACGGGCGTTCCGTCAGTAACCAGTGGGCGGTAGCCTGAAT > NZ_CP009273/601900‑602071 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |