Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	802,549	T→G	T63T (ACA→ACC)	ybhC ←	putative acyl‑CoA thioester hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	802,549	0	T	G	100.0%	113.6 / NA	35	T63T (ACA→ACC)	ybhC	putative acyl‑CoA thioester hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/22);  total (13/22)

CCTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGG  >  NZ_CP009273/802466‑802634                                                                                    |                                                                                      ccTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAgg                                                                                 <  2:1064571/90‑1 (MQ=255) ccTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAgg                                                                                 <  1:677122/90‑1 (MQ=255)   ttGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGa                                                                               <  2:627047/90‑1 (MQ=255)   ttGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGa                                                                               >  1:1412715/1‑90 (MQ=255)   ttGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGa                                                                               <  2:622480/90‑1 (MQ=255)    tGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGAt                                                                              >  2:1070985/1‑90 (MQ=255)     gAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATg                                                                             >  2:316298/1‑90 (MQ=255)      aatGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGc                                                                            <  1:1175909/88‑1 (MQ=255)      aGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGc                                                                            <  2:105832/90‑1 (MQ=255)         gCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCaaa                                                                         >  1:666700/1‑90 (MQ=255)         gCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCaaa                                                                         >  2:362819/1‑90 (MQ=255)           ccgccgGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAAt                                                                       <  1:43637/90‑1 (MQ=255)           ccgccgGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAAt                                                                       <  2:1536331/90‑1 (MQ=255)           ccgccgGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAAt                                                                       <  2:1507308/90‑1 (MQ=255)                          caaAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGa                                                        <  2:326812/90‑1 (MQ=255)                                              aggGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGcc                                    <  2:538447/90‑1 (MQ=255)                                                 gTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGAc                                 <  1:1211533/90‑1 (MQ=255)                                                    aTCGGGGAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGa                              >  1:1530132/1‑90 (MQ=255)                                                       ggggAAGGATTCCACGCTGCAGCCCCCGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATc                           <  2:1467204/90‑1 (MQ=255)                                                       ggggAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATc                           <  1:1298860/90‑1 (MQ=255)                                                       ggggAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATc                           <  2:357333/90‑1 (MQ=255)                                                         ggAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCgg                         >  2:127928/1‑90 (MQ=255)                                                         ggAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCgg                         >  2:1490181/1‑90 (MQ=255)                                                          gAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGAc                                 >  1:1168605/1‑81 (MQ=255)                                                          gAAGGATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGAc                                 <  2:1168605/81‑1 (MQ=255)                                                             ggATTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCga                     <  2:1530132/90‑1 (MQ=255)                                                               aTTCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCgaga                   <  2:1562907/90‑1 (MQ=255)                                                                 tCCACGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGagaag                 <  1:316298/90‑1 (MQ=255)                                                                    aCGCTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAgg              >  2:579214/1‑90 (MQ=255)                                                                       cTGCAGCACCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTAc           >  2:1461113/1‑90 (MQ=255)                                                                         gcagcaCCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCa         >  1:1565391/1‑90 (MQ=255)                                                                             caCCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGgcg     <  2:1565391/90‑1 (MQ=255)                                                                             caCCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGgcg     <  1:651054/90‑1 (MQ=255)                                                                             caCCTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGgcg     <  1:1308291/90‑1 (MQ=255)                                                                                cTGGGGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCgg  >  2:875131/1‑90 (MQ=255)                                                                                    |                                                                                      CCTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGG  >  NZ_CP009273/802466‑802634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑