Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	979,260	T→C	I52T (ATC→ACC)	gloC →	hydroxyacylglutathione hydrolase GloC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	979,260	0	T	C	100.0%	76.8 / NA	25	I52T (ATC→ACC)	gloC	hydroxyacylglutathione hydrolase GloC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/12);  total (13/12)

GCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGCCC  >  NZ_CP009273/979181‑979341                                                                                |                                                                                  gCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgc                                                                              <  2:1003578/85‑1 (MQ=255) gCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCctgc                                                                              >  1:1003578/1‑85 (MQ=255)     cACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATg                                                                     >  1:1102330/1‑90 (MQ=255)      aCTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATgg                                                                    <  1:944391/90‑1 (MQ=255)         ggTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCa                                                                 <  2:1565861/90‑1 (MQ=255)         ggTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGGTGATGACAGCGGCCTGACACTGATCCAGACCCTGCTGACGCATGGTCa                                                                 >  1:1446672/1‑90 (MQ=255)            cGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCt                                                              <  2:1215245/90‑1 (MQ=255)                 cTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGAcc                                                         <  1:957719/90‑1 (MQ=255)                   ggcggcGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCAc                                                       >  1:1439181/1‑90 (MQ=255)                   ggcggcGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCAc                                                       >  2:432217/1‑90 (MQ=255)                      ggcgATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGtt                                                    <  1:965194/90‑1 (MQ=255)                        cgATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTgg                                                  >  1:722563/1‑90 (MQ=255)                                 aaaaaTCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAgcggc                                         <  1:543101/90‑1 (MQ=255)                                          aCAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgc                                <  1:758141/90‑1 (MQ=255)                                            aGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc                              <  1:89222/90‑1 (MQ=255)                                            aGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc                              <  2:1280054/90‑1 (MQ=255)                                            aGGAAGTTGATGACAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc                              <  1:536533/90‑1 (MQ=255)                                                     atgaCAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc                              >  1:53889/1‑81 (MQ=255)                                                     atgaCAGCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGgcgc                              <  2:53889/81‑1 (MQ=255)                                                           gCGGCCTGACACTGATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGc               >  2:782855/1‑90 (MQ=255)                                                                        gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGccc  >  2:1329494/1‑90 (MQ=255)                                                                        gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGccc  >  2:285368/1‑90 (MQ=255)                                                                        gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGccc  >  2:516009/1‑90 (MQ=255)                                                                        gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGGTTTCGGccc  >  2:1152480/1‑90 (MQ=255)                                                                        gATGCAGACCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGGGCCGGGTTTCGGccc  >  2:1218782/1‑90 (MQ=255)                                                                                |                                                                                  GCCGCACTGGTCGATCCTGGCGGCGATGCGGAAAAAATCAAACAGGAAGTTGATGACAGCGGCCTGACACTGATGCAGATCCTGCTGACGCATGGTCATCTGGACCACGTTGGCGCAGCGGCGGAACTGGCGCAACATTACGGCGTGCCGGTTTTCGGCCC  >  NZ_CP009273/979181‑979341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑