Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,153,703	A→T	I127F (ATC→TTC)	ptsG →	PTS glucose transporter subunit IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,153,703	0	A	T	100.0%	134.1 / NA	35	I127F (ATC→TTC)	ptsG	PTS glucose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (19/16);  total (19/16)

CTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATC  >  NZ_CP009273/1153619‑1153789                                                                                     |                                                                                       cTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCa                                                                                   >  1:113477/1‑90 (MQ=255)  tGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAg                                                                                  <  1:1122324/90‑1 (MQ=255)      aCTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTa                                                                              >  1:1302605/1‑90 (MQ=255)      aCTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTa                                                                              <  2:1302605/90‑1 (MQ=255)         gCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACAt                                                                           >  2:722018/1‑90 (MQ=255)             ttACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGttt                                                                       >  2:295742/1‑90 (MQ=255)                   gctgAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGt                                                                 >  2:662644/1‑90 (MQ=255)                   gctgAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGt                                                                 >  1:259151/1‑90 (MQ=255)                   gctgAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGt                                                                 >  1:514650/1‑90 (MQ=255)                    ctgAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGtt                                                                <  1:1304188/90‑1 (MQ=255)                        agaaATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTa                                                            >  1:41350/1‑90 (MQ=255)                         gaaATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTAc                                                           <  2:41350/90‑1 (MQ=255)                         gaaATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTAc                                                           <  2:259151/90‑1 (MQ=255)                             tCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTa                                                       >  1:1570834/1‑90 (MQ=255)                                 ctctAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTaa                                                   <  1:917950/90‑1 (MQ=255)                                       acacCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGcc                                             <  2:737283/90‑1 (MQ=255)                                           cTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAg                                         >  2:931634/1‑90 (MQ=255)                                            tGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGt                                        >  2:125888/1‑90 (MQ=255)                                            tGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGt                                        >  1:366764/1‑90 (MQ=255)                                                  aTACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTg                                  <  2:1103159/90‑1 (MQ=255)                                                       ggCGTCCTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACa                                                                            <  2:1350156/43‑1 (MQ=38)                                                       ggCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACa                                                                            >  1:1350156/1‑43 (MQ=255)                                                       ggCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTAc                                                           >  2:247412/1‑60 (MQ=255)                                                       ggCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTAc                                                           <  1:247412/60‑1 (MQ=255)                                                       ggCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGcttc                             >  1:1180540/1‑90 (MQ=255)                                                       ggCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGcttc                             >  2:904585/1‑90 (MQ=255)                                                                 gAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTa                   >  1:994606/1‑90 (MQ=255)                                                                   gggATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTaaa                 <  2:1161452/90‑1 (MQ=255)                                                                   gggATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTaaa                 <  1:1284051/90‑1 (MQ=255)                                                                   gggATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTaaa                 <  1:777061/90‑1 (MQ=255)                                                                       ttATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCt             >  1:1550963/1‑90 (MQ=255)                                                                         aTCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCttt           <  1:662644/90‑1 (MQ=255)                                                                           ctcCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGcc                                             >  2:555502/1‑54 (MQ=255)                                                                           ctcCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGcc                                             <  1:555502/54‑1 (MQ=255)                                                                                  gCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATc  <  2:1550963/90‑1 (MQ=255)                                                                                     |                                                                                       CTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATC  >  NZ_CP009273/1153619‑1153789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑